Primer sequences and quantitative determination kit used for simultaneously detecting human CMV and BK virus DNA同时检测人CMV和BK病毒DNA的引物序列及定量检测试剂盒本发明公开了可同时用于对巨细胞病毒(CMV)和BK病毒(BKV)脱氧核糖核酸片段(DNA)进行定量检测的引物,荧光探针和实时定量多聚酶链反应(real-time PCR...
LOCUS pRL-CMV 4079 bp DNA SYN DEFINITION pRL-CMV ACCESSION KEYWORDS SOURCE ORGANISM other sequences; artificial sequences; vectors. FEATURES Location/Qualifiers source 1..4079 /organism="pRL-CMV" /mol_type="other DNA" promoter 169..721 /label="CMV_immearly_promoter" misc_feature 224..511 /...
<223>primer-f <400>1 tcgtgaaacacgctcgcttt20 <210>2 <211>20 <212>dna <213>artificialsequence <220> <223>primer-r <400>2 tggtcggtacacggtccctt20 <210>3 <211>20 <212>dna <213>artificialsequence <220> <223>探针 <400>3 gacccggtgttccttggaga20...
pBI-CMV3载体价格2200元,pBI-CMV3载体质粒图谱(Vector map),载体序列(Vector Sequence)见下文,载体质粒抗性为氨苄青霉素,质粒大小为3798bp,测序引物见下文。pBI-CMV3是哺乳动物细胞表达载体pBI-CMV3是双向表达载体。
It also serves as a transcription factor via direct DNA binding at consensus sequence located in the regulatory region of target genes and can serve as an activator or repressor depending on the target gene. The consensus sequence has internal symmetry and consists of two copies of 5′-PuPuPuC...
The sequences of the reference primers were as follows: forward 5′-GGCGGTGGTCCTGGAGTACT-3′, reverse 5′-AGAGGCCTTTGGCTTTCTTCTT-3′, and probe 5′-VIC-ACCCGCCACAAGC-MGB-3′. The lengths of the CMV and POP4 amplicons were 64 bp and 68 bp, respectively. Digital PCR The dPCR ...
Table 6 Primer sequences and amplicon lengths obtained in nested PCR assays in the CMV genes. Full size table Table 7 Restriction enzymes and length of the restriction fragments. Full size table CMV UL128 locus genotyping To successfully amplify the genes encoding the CMV UL128L, nPCR (UL130...
(AgronomyCollegeofYunnanAgriculturalUniversity,Kunming625014;YunnanTobaccoScienceResearchInstitute,YuxiYunnan653100)Abstract:InordertoeffectivelytranscribedsRNAinplants,aninducerofRNAsilencing,thespecificprimerswasdesignedaccordingtothepublishedsequenceofTMVAMPgeneandCMVARepgene.MPandRepfragmentwasobtainedbyPCRamplification...
LOCUS pShuttle-CMV-EGFP-C 8156 bp DNA SYN DEFINITION pShuttle-CMV-EGFP-C ACCESSION KEYWORDS SOURCE ORGANISM other sequences; artificial sequences; vectors. FEATURES Location/Qualifiers source 1..8156 /organism="pShuttle-CMV-EGFP-C" /mol_type="other DNA" misc_feature complement(27..105) /label...
In specimens of one patient, significant underquantification of CMV load with "in-house" PCR emerged during follow-up due to a point mutation in the "in-house" PCR primer sequence. The CAP/CTM CMV test was found suitable for diagnosing and monitoring CMV replication in renal transplant ...