PS酶(PrimerSTARHSDNA) 0.5 10mMdNTP 4.0 5×Buffer 25.0 ddH2O 15.5 反应条件:98℃预变性15s,55℃退火10s,72℃延伸465s,30个循环。 200bp本氏烟PDS保守序列扩增 反应体系如下: 50.0 样品 体积(μl) 模板质粒(pCB301-Fny1) 1.0 F引物(PDS-Sma1-333-F) ...
请帮忙提供 CMV Primer and Control Set 的供应商,距离要近的,到时方便交货。 网友 1 最佳答案 回答者:南京北鱼生物科技有限公司 我们可以提供如下规格: 产品名称:CMV Primer and Control Set/品牌:Norgen Biotek/产地:美国点击查看产品详情 联系电话:19852887912 QQ:2245068322我来回答 提交回答 重置等你来答 有...
PROBLEM TO BE SOLVED: To obtain the subject new nucleic acid for primer, etc., containing a nucleic acid sequence comprising continued bases having specific numbers among specific nucleic acid sequences and a promoter sequence and capable of rapidly, simply and specifically proliferating and detecting...
The development of a quadriplex PCR method with amplification of HCMV in a single-step procedure using primers taken from four different regions of the vir... P Markoulatos,V Samara,N Siafakas,... - 《Journal of Clinical Laboratory Analysis》 被引量: 10发表: 2015年 Transfection of bone ...
pFlag-CMV-2质粒图谱
(构建pCB301-Fny1-3Um突变体) 反应体系如下: -20℃保存样品体积(μl)模板质粒(pCB301-Fny1) 1.0 F引物(Fny1-BSS-3151-F) 2.0 R引物(Fny1-B-3150-R) 2.0 PS酶(PrimerSTAR HS DNA) 0.5 10mM dNTP 4.0 5×Buffer 25.0 ddH2O 15.5 50.0 反应条件:98 ℃预变性15s,55 ℃退火10 s,72 ℃延伸465s,...