Reagent A consists of a set of a mixture of primers and probes, primers and probes are directed to a conserved sequence of CMV DNA, BKV DNA; the reagent B containing dNTP buffer; reagent C for Taq DNA polymerase; Reagent D by different concentrations of the standard product composition; ...
The sequences of the reference primers were as follows: forward 5′-GGCGGTGGTCCTGGAGTACT-3′, reverse 5′-AGAGGCCTTTGGCTTTCTTCTT-3′, and probe 5′-VIC-ACCCGCCACAAGC-MGB-3′. The lengths of the CMV and POP4 amplicons were 64 bp and 68 bp, respectively. Digital PCR The dPCR ...
The sequences of the primers are as follows: p35 forward, 5′-GGAATTCTATGTGTCAATCACGCTACCTCC-3′; p35 backward, 5′-CCCCCCGGGCGATCAGGCGGAGCTCAGATAGCCC-3′; p40 forward, 5′-GGAATTCTATGTGTCCTCAGAAGCTAACCA-3′; p40 backward, 5′-CCCCCCGGGCGACTAGGATCGGACCCTGCAGGGA-3’ (EcoRI and X...
Gene Sequences| Promoter|CMV T-terminal Tag|N/A C-terminal Tag|His Cloning Method|Restriction Enzyme 5'→3' Cloning Site|NheⅠ/XhoⅠ 5′ sequencing primer|forward primer 5‘-GGTCTATATAAGCAGAGCTG-3´ 3′ sequencing primer|Reverse primer 5’-GTGGTATGGCTGATTATGATCAG-3´...
Gene Sequences| Promoter|CMV T-terminal Tag|N/A C-terminal Tag|Flag Cloning Method|Restriction Enzyme 5'→3' Cloning Site|NheⅠ/XhoⅠ 5′ sequencing primer|forward primer 5‘-GGTCTATATAAGCAGAGCTG-3´ 3′ sequencing primer|Reverse primer 5’-GTGGTATGGCTGATTATGATCAG-3´...
DNA was analyzed by quantitative real-time polymerase chain reaction (RT-PCR) using a primer and probe set directed against the HCMV US28 gene sequence: probe, TGA TCC CGC TCA GTG T; forward primer, GAA CTC ATG CTC GGT GCT TTC; reverse primer, CTT TGT GGC GCG ACT GAG A. This primer...
sequencing_primer_5CMV forward site_3_destroyed site_5_destroyed entrez_gene aliases geneRheb id26954 genbank_ids mutationD60I to make dominant negative nameDominant negative Rheb (D60I) shRNA_sequence size species 10116 Rattus norvegicus
pFLAG-CMV2载体价格1500元,pFLAG-CMV-2载体质粒图谱(Vector map),载体序列(Sequence)见下文,载体质粒抗性为Ampicillin,质粒大小为4679bp,测序引物为CMV-F和hGH-pA-R,是真核表达系统的pFLAG-CMV2载体。
N-CMV-30 Forward Sequencing Primer (P5350) 1 μg ANTI-FLAG M2 Monoclonal Antibody (F3165) 200 μg ANTI-FLAG M5 Monoclonal Antibody (F4042) 200 μg ANTI-FLAG M2 Affinity Gel - Freezer Safe (A2220) 1 ml Met-FLAG-BAP Control Protein (P5975) 100 ug ...
Gene Sequences| Promoter|CMV T-terminal Tag|N/A C-terminal Tag|EGFP Cloning Method|Restriction Enzyme 5'→3' Cloning Site|NheⅠ/XhoⅠ 5′ sequencing primer|forward primer 5‘-GGTCTATATAAGCAGAGCTG-3´ 3′ sequencing primer|Reverse primer 5’-GTGGTATGGCTGATTATGATCAG-3´...