Diagnosis of cy- tomegalovirus (CMV) disease in renal allograft recipients: The role of semi-quantitiative Polymerase Chain Reaction (PCR). Neph Dial Trans 1995;10:1198 - 205.Peiris, J. S. M., Taylor, C. E., Main, J., Graham, K., Madeley, C. R. (1995) Diagnosis of ...
刘庆 , 王继东, 吉耀华 , 阮强 摘要: 目的用 FQ— PCR 、 CLIA 法检测尿液 HCMV. DNA 及血清HCMV-IgM 抗体水平。结果MV. ]si 抗体阳性 206 例; 二者均阳性 191 例。478 例尿液 HCMV— DNA 阳性者中, 血清 lgM抗体阳性 HCMV·DNA 拷贝数对数均值高于阴性者 (P < 0. 01) 。结论尿液 HCM~·...
DNA was extracted from separated CD4+CD45RA+ T cells using QIAamp DNA Mini Kit (Qiagen, Chatsworth, California, USA). In order to remove contaminations, which would interfere with polymerase chain reaction (PCR), DNA was purified by ethanol-precipitation using 0.4M LiCl2 and 2.5-fold the ...
[7] ont quant fi eux retrouv6 des antig6nes d'HSV-2 en IFI sur 25 % des coupes de prostate qu'ils ont exa- min6es. L'existence d'un r6servoir de virus au niveau du tractus g6nital male a 6t6 confir- m6e par les donn6es exp6rimentale [18]. I1 a 6t6 montr6 que l'...
For plasma quality controls, both PCR assays are correlated VQC (R 2 = 0.93). On whole blood or infected cells dilutions, correlation shows an overestimation by the LC1UL83 assay (mean 1.2 log copies/ml) over 3 log though R 2 = 0.94. Results with CMV Quant Kit are closer to ...
Methods This retrospective case series included adult ALL patients (>14 years) who were being treated with chemotherapy between July 2009 to July 2011 at a tertiary care centre and detected to have CMV viraemia (Real time quantitative PCR with Roche CMV DNA QuantKit). PCR was done in patients...
DNAemiapp65TransplantationThe performance of two commercially available CMV pp65 antigenemia (AG) assays (CINA kit, Argene Biosoft, CK, and CMV Brite kit, IQ Products, CBK), and a quantitative PCR test (COBAS Amplicor CMV Monitor Test, Roche Diagnostics, CACM) was evaluated with 667 blood ...
2.3. Viral Load Determination by qPCR PCR testing of DBS DNA was performed in triplicate, as previously described [19]. Multiplex qPCR was performed using the UL83 and NRAS primers shown in Table 1. The standard curve for NRAS was generated using five 10-fold dilutions starting with 200,00...