For plasma quality controls, both PCR assays are correlated VQC (R 2 = 0.93). On whole blood or infected cells dilutions, correlation shows an overestimation by the LC1UL83 assay (mean 1.2 log copies/ml) over 3 log though R 2 = 0.94. Results with CMV Quant Kit are closer to ...
DNA was extracted from separated CD4+CD45RA+ T cells using QIAamp DNA Mini Kit (Qiagen, Chatsworth, California, USA). In order to remove contaminations, which would interfere with polymerase chain reaction (PCR), DNA was purified by ethanol-precipitation using 0.4M LiCl2 and 2.5-fold the ...
Seventy-seven renal allograft recipients were enrolled in a prospective study where CMV viruria (shell vial culture/DEAFF test), viraemia (shell vial culture), serology and detection of virus DNA in peripheral blood leukocytes by PCR (CMV DNAemia) were correlated with clinical evidence of CMV ...
DNAemiapp65TransplantationThe performance of two commercially available CMV pp65 antigenemia (AG) assays (CINA kit, Argene Biosoft, CK, and CMV Brite kit, IQ Products, CBK), and a quantitative PCR test (COBAS Amplicor CMV Monitor Test, Roche Diagnostics, CACM) was evaluated with 667 blood ...