Molecular methods such as polymerase chain reaction (PCR) are used to diagnose congenital infections in newborns and may be used to detect and/or confirm active infections in others. These methods may be used to detect the genetic material (DNA) of CMV (qualitative testing) and/or measure the...
Amniocentesis wascarried out to determine the PCR specific for viral DNA detection.Results of this test and of ultrasound monitoring were confrontedwith the immediate perinatal result, and viral cultures in newbornurine were performed using human fibroblasts, the gold standard fordiagnosis of congenital...
PCRBackgroundThere is a growing need for sensitive high-throughput cytomegalovirus (CMV) PCR tests due to the increasing number of immunocompromised patients requiring monitoring for active CMV infection.ObjectivesTo compare the fully automated COBAS AmpliPrep/COBAS TaqMan (CAP/CTM) CMV test (this ...
目的探讨人巨细胞病毒(HCMV)感染与冠心病的关系及HCMV感染可否作为冠心病的独立危险因子.方法间接酶联免疫吸附试验(ELISA)测定HCMV-IgG、HCMV-IgM和HCMV-IgA抗体,聚合酶链反应(PCR)测定HCMV-DNA.结果冠心病组的HCMV-IgG、IgM、IgA抗体阳性率、S/N值(标本450 nm吸光度值/阴性对照450nm吸光度值)和HCMV-DN...
However, 11 of the 20 infants did not pass a second hearing screen and had urine CMV PCR testing, and 1 (9%) was positive. This latter infant's newborn dried blood spot CMV DNA PCR was negative so a diagnosis of congenital CMV infection was not possible. Targeted screening in the ...
方法:采用德国Virion/serion公司酶联免疫吸附试验(ELISA)定量试剂,检测孕妇血清中HCMV的IgM和IgG抗体、IgG抗体亲和力;采用中山医科大学达安公司RV核酸扩增(PCR)荧光定量检测孕妇血清中HCMV-DNA,并对其结果进行分析。结果:341例孕妇中,HCMV-IgG低亲和力样本孕产史不良孕妇组所占比例显著高于初次妊娠孕妇。孕产史不良孕妇...
方法:采用德国Virion/serion公司酶联免疫吸附试验(ELISA)定量试剂,检测孕妇血清中HCMV的IgM和IgG抗体、IgG抗体亲和力;采用中山医科大学达安公司RV核酸扩增(PCR)荧光定量检测孕妇血清中HCMV-DNA,并对其结果进行分析。结果:3 2、41例孕妇中,HCMV-IgG低亲和力样本孕产史不良孕妇组所占比例显著高于初次妊娠孕妇。孕产史...
大部分感染CMV的孕妇,约需4个月时间形成高亲和力抗体,如果该孕妇处于妊娠早期,IgG亲和力高,可以判断CMV感染时间是在怀孕之前,因此对胎儿危害小。如果抗体亲和力低,则约有40%的几率会将病毒传染给胎儿,需通过羊膜穿刺术,羊膜穿刺PCR等方法进一步检查,以明确病毒...
摘要: Cytomegalovirus (CMV) DNA detection in blood could, as a supplementary test to serology, improve the accuracy and speed of diagnosis of an acute CMV infection. In this study we evaluated the performan关键词: RealStar Immunocompetent Patients Primary Human Cytomegalovirus Altona Diagnostics ...
本发明提供了一种用于诊断人巨细胞病毒感染的试剂,包括:人巨细胞病毒DNA PCR扩增引物,miRNA-UL112-3p正向PCR扩增引物,以及miRNA-UL112-5p正向PCR扩增引物. The present invention provides reagents for diagnosis of human cytomegalovirus infection include: human cytomegalovirus DNA PCR primers, miRNA-UL112-3p fo...