These methods may be used to detect the genetic material (DNA) of CMV (qualitative testing) and/or measure the amount of viral DNA in a sample (quantitative, also called viral load). Molecular methods are more sensitive than culture, and positive and negative results are available in a ...
Quantitative detection of viral DNA in milk. Viral DNA load (copies/mL) was determined by quantitative PCR (CO- BAS Amplicor CMV Monitor Test; Roche Molecular Bio- chemicals, Mannheim, Germany) using milk whey from natu- rally infected milk and spiked milk after virus inoculation (13). ...
Testing for NDV-replication by quantitative (q)-PCR, showed marked increase in NDV-replication in cells transfected with UL48N but not with UL48NΔDUB (Figure 4j). This finding suggests that UL48N suppressed cellular anti-viral responses, which was further proved by analyzing the replication ...
For the control group, group, DNA was extracted from 200 μl urine using a NucliSens easyMAG (bioMérieux) according to manufacturer guidelines. The Specific A protocol was used with a final elution volume of 70 μl. Quantitative CMV PCR (qPCR) CMV viral titers (copies/ml) were quantified...
Quantitative PCR is the method of choice for determining telomere length in small extractable quantities of DNA, as is the case in our study. Due to limited sample size not all laboratory investigations regarding quantification of lymphocytes, RTL or TRECs could be performed in all patients. In ...
the use of the Delphi methodology as a structured expert elicitation technique enabled the capture and interpretation of a broad spectrum of perspectives, opinions, and treatment practices. This methodology facilitated an in-depth exploration of attitudes and opinions not possible in quantitative surveys....
Bei Transplantationspatienten wird zum Monitoring der Reaktivierung der quantitative Nachweis von pp65- Antigen in neutrophilen Granulozyten, neuerdings vermehrt der quantitative HCMV-DNA-Nachweis aus Vollblut oder Plasma, durchgeführt. Bei einer HCMV- Enzephalitis kann HCMV-DNA im Liquor nach...
PCR with FastStart Taq DNA polymerase (Roche) was performed according to factory supplier using degenerate broad primers specific for 50 UTR ends of heavy and kappa mouse V genes and specific IgG constant and kappa constant 30 primers67. Purified PCR product was then submitted for Sanger ...
2.7. HCMV PCR Assay Quantitative CMV DNA PCR was performed using a standardized commercially available COBAS®AmpliPrep/COBAS®TaqMan® CMV Test for fully automated specimen processing, amplification, and detection (Roche Molecular Systems, Mannheim, Germany), which had been calibrated and showed ...
Detection of virus nucleic acids in glioblastoma tissue samples by PCR or sequencing methods is however also difficult [20,21,26,29,30], although some investigators have detected HCMV DNA/RNA in glioblastoma tumors using PCR [1,11,31], or in situ hybridization techniques [19]. It is not ...