Quantitative detection of viral DNA in milk. Viral DNA load (copies/mL) was determined by quantitative PCR (CO- BAS Amplicor CMV Monitor Test; Roche Molecular Bio- chemicals, Mannheim, Germany) using milk whey from natu- rally infected milk and spiked milk after virus inoculation (13). ...
Plasma PCR for cytomegalovirus DNA after allogenic marrow transplantation: comparison with PCR using peripheral blood leukocytes, pp65 antigenemia, and viral culture Transplantation (1997) A. Ferreira-Gonzalez et al. Clinical utility of a quantitative polymerase chain reaction for diagnosis of cytomegalovirus...
Quantitative PCR is the method of choice for determining telomere length in small extractable quantities of DNA, as is the case in our study. Due to limited sample size not all laboratory investigations regarding quantification of lymphocytes, RTL or TRECs could be performed in all patients. In ...
Quantitative PCR would help to determine the declining or rising of viral load that could be important for pre-emptive antiviral therapy. In the present study, there was no biopsy available for the CMV DNA detection to confirm the diagnosis of end organ- disease. Therefore, the clinical ...
desquantitativen CMV-DNA-Nachweises fuÈr klinischeZwecke zur verbesserten Behandlung und UÈberwa-chung von Risikopatienten.SchluÈsselwoÈrter:Virus-Quantifizierung; Zyto-megalievirus (CMV); virale NukleinsaÈuren; antiviraleMedikamente; Polymerase-Kettenreaktion (PCR).Summary:The development...
These methods may be used to detect the genetic material (DNA) of CMV (qualitative testing) and/or measure the amount of viral DNA in a sample (quantitative, also called viral load). Molecular methods are more sensitive than culture, and positive and negative results are available in a ...
Bei Transplantationspatienten wird zum Monitoring vor allem der quanti- tative HCMV-DNA-Nachweis (NAT) aus Vollblut oder Plasma durchgeführt. Der quantitative Nachweis von pp65- Antigen in Leukozyten ist wegen man- gelnder Standardisierbarkeit, geringe- rer Sensitivität gegenüber NAT ...
CMV reactivation is defined as the initial demonstration of CMV infection either by blood culture (BC) or by 2 consecutive quantitative serum polymerase chain reaction (PCR) assays of CMV DNA during a monitoring period that began at day 21 post-HCT and continued thereafter twice weekly until day...
Of the individuals with TCR-seq data, 50% of HIV-positive non-diabetics had detectable HIV in adipose tissue by quantitative PCR, whereas 75% of HIV-positive diabetics had detectable virus. There were a few shared/public TCRs with identical CDR3 amino acid sequences on CD4+ T cells from ...
RT-PCRReal-time quantitative polymerase chain reaction Fc receptorsReceptors for the constant regions of their H chain TEMRATerminally differentiated T-cell NMDPUS National Marrow Donor Program VZVVaricella-zoster virus References Wróbel, M.J.; Greczka, G.; Szyfter, W. The risk factor profile of...