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For plasma quality controls, both PCR assays are correlated VQC (R 2 = 0.93). On whole blood or infected cells dilutions, correlation shows an overestimation by the LC1UL83 assay (mean 1.2 log copies/ml) over 3 log though R 2 = 0.94. Results with CMV Quant Kit are closer to ...
Quant itative PCR( FQ-PCR )and LIAISON Chemilumineseent Imma n oassay ( CLIA) respectively. R esults 478 cases were posit i ve f o r H C MV . D NA . and 206 cases were positive for H CM V . Ig M . 19 1 ca s es were posi tive for two indexes.T he viral DNA load(...
(B) Viral load by quantitative PCR from 4 dpi spleen DNA. Data point marked by asterisk is identified as an outlier by a Grubb outlier test with α < 0.05; p value reaches significance with the outlier so an additional p value omitting the value is not listed. (C) Mouse weight loss ...
Reagent mixture composition shared by T and S PCR was 15 mM Tris–HCl (pH 8.0), 50 mM KCl, 200 μM dNTP, 1% DMSO, 2.5 mM DTT, SYBR-Green I. Composition specific for the T PCR was 1.5 mM MgCl2, 1.5 Units AmpliTaq Gold DNA polymerase and 450 nM of each telomere specific primer....
7.根据权利要求4所述的三重实时荧光定量PCR试剂盒,所还包括病毒DNA提取试剂。 8.根据权利要求4所述的三重实时荧光定量PCR试剂盒,还包括荧光实时定量PCR扩增所需的PCR缓冲液、Taq酶和dNIPs。 9.根据权利要求8所述的三重实时荧光定量PCR试剂盒,所述引物、荧光探针、PCR缓冲液、Taq酶和dNIPs混合呈PCR预混液。 说...
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Methods This retrospective case series included adult ALL patients (>14 years) who were being treated with chemotherapy between July 2009 to July 2011 at a tertiary care centre and detected to have CMV viraemia (Real time quantitative PCR with Roche CMV DNA QuantKit). PCR was done in patients...
DNAemiaThe performance of two commercially available CMV pp65 antigenemia (AG) assays (CINA kit, Argene Biosoft, CK, and CMV Brite kit, IQ Products, CBK), and a quantitative PCR test (COBAS Amplicor CMV Monitor Test, Roche Diagnostics, CACM) was evaluated with 667 blood samples from 215 ...
Diagnosis of cy- tomegalovirus (CMV) disease in renal allograft recipients: The role of semi-quantitiative Polymerase Chain Reaction (PCR). Neph Dial Trans 1995;10:1198 - 205.Peiris, J. S. M., Taylor, C. E., Main, J., Graham, K., Madeley, C. R. (1995) Diagnosis of ...