Here, we address fundamental questions about the closed-loop model and discuss how a growing understanding of mRNA structure, dynamics, and intermolecular interactions presents new experimental opportunities. We anticipate that the application of emerging methods will lead to expanded models that include ...
In eukaryotic translation the 60S ribosome subunit has not been proposed to interact with mRNA or closed-loop factors eIF4E, eIF4G, and PAB1. Using analytical ultracentrifugation with fluorescent detection system, we have identified a 57S translation complex that contains the 60S ribosome, mRNA, and...
miRNA Effects on mRNA closed-loop for- mation during translation initiation. Prog Mol Subcell Biol 2010; 50: 99-112.Beilharz, T. H., Humphreys, D. T., and Preiss, T. (2009) miRNA effects on mRNA closed-loop formation during translation initiation. In: Rhoads R. E. (ed.), miRNA ...
Artificial intelligence-guided closed-loop experimentation has emerged as a promising method for optimization of objective functions1,2, but the substantial potential of this traditionally black-box approach to uncovering new chemical knowledge has remained largely untapped. Here we report the integration ...
Artificial intelligence-guided closed-loop experimentation has emerged as a promising method for optimization of objective functions, but the substantial potential of this traditionally black-box approach to uncovering new chemical knowledge has remained largely untapped. Here we report the integration of cl...
Here we overcome these issues by developing a flexible bioelectronic system consisting of wirelessly powered, closed-loop sensing and stimulation circuits with skin-interfacing hydrogel electrodes capable of on-demand adhesion and detachment. In mice, we demonstrate that our wound care system can ...
Machine learning (ML) has emerged as a vital component in designing these closed-loop systems as ML models can predict / identify presence of disease symptoms based on neural activity and adaptively learn to modulate stimulation. We queried the US National Library of Medicine PubMed database to ...
(TIB-Molbiol, Berlin), as shown in SEQ ID NO: 5, were heated to a temperature of 90° C. for 5 minutes and subsequently cooled on ice to enable development of a stem-loop structure. Self-complementary overhangs of such ODNs were ligated with a final concentration of 1 μg/μl DNA...
Stem-loop or hairpin adapters are single-stranded, but their termini are complementary such that the adapter folds back on itself to generate a double-stranded portion and a single-stranded loop. A stem-loop adapter can be linked to an end of the linear, double-stranded nucleic acid molecule...
14. The method of claim 1, wherein the isothermal detection methods is by strand displacement amplification, rolling circle amplification, loop-mediated isothermal amplification, isothermal chimeric primer-initiated amplification of nucleic acids, Q-beta amplification systems or OneCutEventAmplificatioN. 15...