Stem-Loop Structure Any DNA or RNA that may have non-paired single strand loops associated with a double-strand stem similar to a lollipop (see Fig.S118). In the stem, the Watson-Crick pairing may not be perfect along its length.palindrome,repeat inverted...
Our study provides a molecular basis of this RNA recognition using a combination of solution-state NMR spectroscopy, calorimetry, docking and molecular dynamics simulation. Analysis of TAF15-RRM solution structure and its binding with stem-loop RNA has yielded conclusive evidence of a non-canonical ...
U6 small nuclear RNA is one of the spliceosomal RNAs involved in pre-mRNA splicing. In the fission yeast Schizosaccharomyces pombe, the U6 RNA gene was fou... Tani,Ohshima - 《Genes & Development》 被引量: 69发表: 1991年 The ribozyme core of group II introns: a structure in want of pa...
The spatial constraint of the stem-loop structure of the modified RT primer allowed detection of sncRNAs directly from cell lysates without laborious total RNA isolation, and the poly(U) tail made it possible to use multiplex RT reactions of mRNA and sncRNAs in the same run (Mei et al., ...
genomic RNA into a double-shell structure. One of these shells of RNA density is closely associated with the CP dimers within the protein capsid. This averaged RNA density occupies the approximate position of the TR stem–loop with respect to each dimer, but the averaged density below A/B an...
Hairpin or stem-loop is a commonly observed secondary structure of RNA where A, U, G, C nucleotides form hydrogen bonds with complementary nucleotides on a wrinkled or folded back segment of the same strand (Svoboda & Cara, 2006; From:Methods in Microbiology,2021 ...
FIG. 2B depicts a model of the secondary secondary structure of full length hybrid gRNA. FIG. 2B discloses SEQ ID NO: 37. The gRNA sequence was tested in silico for the formation of a loop structure using M-fold RNA secondary structure analysis tool. The hybrid gRNA depicted is 124 ...
We have probed the structure of the telomerase RNA in the reconstituted complex with RNases T1 and V1. Upon TERT binding to the RNA, sites of both protection and enhancement of cleavage were observed, suggesting potential protein-binding sites and conformational changes in the RNA. Especially ...
Atg16l1 in situ structures show that it strengthens the interaction between TIR domain of TLR7 and MyD88 through specific stem-loop structure changes as a molecular scaffold after TLR7 activation to promote TLR7 downstream signaling. Therefore, we discover a mechanism for host RNA regulation of ...
The HIV-1 transcript is alternatively spliced to over 30 different mRNAs. Whether RNA secondary structure can influence HIV-1 RNA alternative splicing has not previously been examined. Here we have determined the secondary structure of the HIV-1/BRU RNA segment, containing the alternative A3, A4a...