fragilis and Escherichia coli were constructed and used as molecular cloning vectors. The 2.7-kilobase pair (kb) cryptic Bacteroides plasmid pBI143 and the E. coli clon... CJ Smith - 《Journal of Bacteriology》 被引量: 116发表: 1985年 Interactions of the QacR Multidrug-Binding Protein with ...
It is therefore the principal object of the present invention to provide a new class of plasmid cloning vehicles incorporating not only the promoter and 5'-untranslated region, but also the 3'-untranslated region and the transcription termination site, derived from a particular class of bacterial g...
A large number of cloning vectors are available. But, choosing the vector may depend upon a number of factors, such as the size of the insert, copy number and cloning method. Once a gene has been cloned there is almost no limit to the information that can be obtained about its structure...
hs using conjugative or mobilizable cloning vectors. The former are transferred between bacterial cells by simple mating techniques, and the latter are transferred only with the assistance of another mobilizing plasmid that codes for the gene products necessary for conjugal transfer. Those vectors ...
Restriction-modification systems are a diverse class of enzymes. They are classified into four major types: I, II, III and IV. We have previously proposed the existence of a Thermus sp. enzyme family, which belongs to type II restriction endonucleases (R
The present invention relates to compositions including: (1) isolated DNA encoding the StuI restriction endonuclease and isolated DNA encoding cognate and non-cognate methylase; (2) vectors and cells containing the isolated DNA; and (3) methods for producing the StuI restriction endonuclease. ...
Sequence comparison is essential for the analysis of clones and constructed vectors. It is usually performed using Basic Local Alignment Search Tool (BLAST) (Altschul et al., 1990). However, BLAST outputs do not usually show DNA sequence features, making it a time-consuming process to identify ...
The constructed recombinant vectors, pCmER10 and pGSTCmER, were introduced into E. coli BL21 (DE3) and E. coli BL21 cells, respectively. Transformant cells were selected on LB-agar plates containing appropriate antibiotics and cultured in LB media at 37°C with vigorous shaking. Expression of...
type IIG restriction enzymes possess relatively low specific activity so detection of an active enzyme within a cellular extract may be a limiting factor in the isolation of a recombinant clone. AcuI over-expression was attempted from a total of three vectors. Finally, a wild-type recombinant AcuI...
Baculovirus vectors repress phenobarbital-mediated gene induction and stimulate cytokine expression in primary cultures of rat hepatocytes. Gene Ther. 2000, 7, 1274–1283. [Google Scholar] [CrossRef] Sekar, N.; Veetil, S.K.; Neerathilingam, M. Tender coconut water an economical growth medium ...