RNA结合蛋白(RBP)是一类与RNA直接相互作用的蛋白质。 Crosslinking and Immunoprecipitation(CLIP)是基于交联的免疫沉淀分析RBP与靶分子的准确结合位置的实验技术。其技术原理是紫外照射下RNA与RNA结合蛋白发生偶联,然后使用RNA结合蛋白的特异性抗体,通过免疫沉淀的方式富集RNA-蛋白质复合物,同时结合RNase消化的方式去除未被...
440037:36 CLIP Kit 实验操作 伯信生物· 2022-5-20 472001:52 CLIP-seq技术介绍 武汉瑞兴生物· 2023-4-14 1005035:05 嘉因生物-ATAC-seq实验原理及应用 表观遗传上海嘉因· 2022-4-8 3083004:08 实验指南:Stereo-seq 时空组学组织包埋指南(新鲜样本) 华大时空· 2022-10-13 82521016:05 【陈巍学基因】第...
CLIP Tool Kit (CTK): a flexible and robust pipeline to analyze CLIP sequencing data. Bioinformatics 33, 566–567 (2017). CAS PubMed Google Scholar Haberman, N. et al. Insights into the design and interpretation of iCLIP experiments. Genome Biol. 18, 7 (2017). Article PubMed PubMed ...
Discovery of interaction sites between RNA-binding proteins (RBPs) and their RNA targets plays a critical role in enabling our understanding of how these RBPs control RNA processing and regulation. Cross-linking and immunoprecipitation (CLIP) provides a
1B(d)). The RNAs isolated from these complexes were used as templates to generate a stranded library by using the Small RNA expression Kit (SREK, SoliD) (Fig. 1B(e)) for SoliD sequencing. 3.2. Sequence data and treatment Two different libraries of CELF1 CLIP-seq (libA and libB) ...
紫外交联免疫共沉淀测序(CLIP seq)即紫外交联免疫沉淀结合高通量测序(crosslinking-immunprecipitation and high-throughputsequencing),是一项在全基因组水平揭示RNA分子与RNA结合蛋白相互作用的革命性技术。其主要原理是基于RNA分子与RNA结合蛋白在紫外照射下发生耦联,以RNA结合蛋白的特异性抗体将RNA-蛋白质复合体沉淀之后,...