RNA结合蛋白(RBP)是一类与RNA直接相互作用的蛋白质。 Crosslinking and Immunoprecipitation(CLIP)是基于交联的免疫沉淀分析RBP与靶分子的准确结合位置的实验技术。其技术原理是紫外照射下RNA与RNA结合蛋白发生偶联,然后使用RNA结合蛋白的特异性抗体,通过免疫沉淀的方式富集RNA-蛋白质复合物,同时结合RNase消化的方式去除未被...
Discovery of interaction sites between RNA-binding proteins (RBPs) and their RNA targets plays a critical role in enabling our understanding of how these RBPs control RNA processing and regulation. Cross-linking and immunoprecipitation (CLIP) provides a
1B(d)). The RNAs isolated from these complexes were used as templates to generate a stranded library by using the Small RNA expression Kit (SREK, SoliD) (Fig. 1B(e)) for SoliD sequencing. 3.2. Sequence data and treatment Two different libraries of CELF1 CLIP-seq (libA and libB) ...