protein cleavage reactions can be performed under conditions that do not affect the activity or stability of the target protein. This product includes a Cleavage Control Fusion Protein. The Control Protein has a N-terminal 6xHN tag. Digestion of the Control Protein with HRV 3C Protease results in...
3. At doses of 4.5 and 9 micrograms kg-1 OSW-1, the serum oestradiol (E2) levels at the expected next pro-oestrus were significantly lower than in control (pro-oestrus). The serum luteinizing hormone (LH) levels 4 days after 9 micrograms kg-1 OSW-1 treatment were also markedly lower...
产品信息 Components • 50 U Tag•Off™ rEK Cleavage Capture Kit • 10 µg Cleavage Control Protein • 2 ml 1X rEK Dilution/Storage Buffer • 5 ml 10X rEK Cleavage/Capture Buffer • 1.5 ml EKapture™ Agarose • 10 Spin Filter, 2 ml ...
Ataxin-3 with a deletion of the previously identified calpain cleavage site (ΔL255-G260) was used as a control protein. There is a rapid decrease in full length protein and appearance of shorter protein fragments with increasing concentrations of calpain-2. After calpain-2 incubation, ...
A full understanding of the mechanism of sterol regulation requires a more complete structural and functional analysis of SCAP, the protein that mediates this control. Studies to date have shown that SCAP comprises two distinct domains: an NH2-terminal polytopic membranous domain of ∼730 amino aci...
Two step reverse transcription-polymerase chain reactions (RT-PCR) were carried out, which amplified 805 base pair (bp) of the first part, including the cleavage site, of the fusion protein gene (F gene) of NDV strains using a set of gene specific primers. Following sequencing, alignment ...
Protein amounts were either roughly adapted to PrP expression (I) or normalized for total protein (II). Actin: loading control, ADAM10 levels are also shown in II. # indicates an unspecific band detected with sPrPY226 Full size image Altered distribution of sPrP with accumulation in ...
control plasmid encoding gene construct for His6-eSrtA-LPETG-MT3 (eSrtA-MT3 without SUMO) fusion protein was also generated by cloning eSrtA-LPETG-MT3 gene between BamHI and XhoI sites of a pET28-TCT universal plasmid (data not shown). The information about this control fusion protein'...
Figure 7 | PLK1 control for dual functions of PCNT. (a) HeLa cells were transiently transfected with the wild-type and mutant FLAG-PCNT-Myc (WT, AA, 9A and RA), arrested at M phase and forced to exit mitosis with ZM447439 (ZM). Immunoblot analyses were performed to determine specific ...
Here, we used the same cell fusion system (Fig. 3d) to evaluate the effect of CTSL on FM-mutants-mediated syncytium formation. FM-ARAA PsV induced a low level of syncytium formation similar to that of the control group (Fig. 3h, i). However, after CTSL was added to the mutant ...