Preferably, in the vector, the Factor Xa cleavage site coding sequence is fused at one end to a product and at its other end to an ATG codon or a sequence coding for at least part of a host protein. This invention also provides a process, for the production of a desired protein or ...
Subbiah M, Khattar SK, Collins PL, Samal SK (2011) Mutations in the fusion protein cleavage site of avian paramyxovirus serotype 2 increase cleavability and syncytium formation but do not increase viral virulence in chickens . J Virol 85 : 5394–5405...
The amino acid sequence at the protease cleavage site of the fusion (F) protein has been postulated as a major determinant of NDV virulence. In this study, we have examined the role of F protein cleavage site sequence in NDV virulence by use reverse genetics technology. The sequence G-R-Q...
It has now been determined that it is possible to form a fusion protein of the epitope with a protein to be isolated, and isolate the protein using HPC-4-based affinity chromatography. In the preferred embodiment, a specific protease cleavage site is inserted between the epitope and the ...
It has now been determined that it is possible to form a fusion protein of the epitope with a protein to be isolated, and isolate the protein using HPC-4-based affinity chromatography. In the preferred embodiment, a specific protease cleavage site is inserted between the epitope and the ...
e.Fusion Proteins and Protein Cleavage Multifunctionalfusion proteinsare a good choice for co-expression provided that the domains of interest are active in similarsubcellular localizations(Fig. 11E). Some cytosolic proteins might also function when expressed as the cytoplasmic tail of a membrane-anchore...
protein can be separated from the fusion protein by, for example, cleaving the fusion protein using any suitable approach or method, including but not limited to cleavage at a proteolytic cleavage site engineered to separate the RBP and the target protein. In certain approaches a cell culture ...
Unlike these viruses, cleavage of the NiV F protein is ubiquitous and does not require the addition of exogenous proteases in cell culture. To determine the importance of the amino acid sequence at the NiV F protein cleavage site for ubiquitous activation, we generated NiV F proteins with ...
Chemical cleavage of fusion proteins for high-level production of transmembrane peptides and protein domains containing conserved methionines. Biochim. Biophys. Acta 1778 (4), 1060-1066.Jian Hu,Huajun Qin,Mukesh Sharma,Timothy A. Cross,Fei Philip Gao.  Chemical cleavage of fusion proteins for...
Deduced amino acid sequences at the fusion protein cleavage site of Newcastle disease viruses showing variation in antigenicity and pathogenicity The amino acid sequence at the F2/F1 cleavage site of the F0 fusion protein of 17 strains of Newcastle disease virus (NDV) was deduced from sequencing ...