Using metabolic and pathway inhibitors, we demonstrate that ceramide is internalized through endocytic pathways and is primarily targeted into perinuclear/endoplasmic reticulum membranes. Further investigations suggested that Giardia uses both clathrin-dependent pathways and the actin cytoskeleton for ceramide ...
A clathrin-dependent pathway leads to KRas signaling on late endosomes en route to lysosomes.Ras proteins are small guanosine triphosphatases involved in the regulation of important cellular functions such as proliferation, differentiation, and apoptosis. Understanding the intracellular trafficking of Ras ...
While Vps10p follows a direct pathway between the TGN and the late endosome/prevacuolar compartment (PVC), Kex2p partitions between this pathway and an indirect pathway through the early endosome. Using cell-free reconstitution of TGN-PVC transport [Abazeed, M.E., et al. (2005) 280:4442鈥 ...
Hoekstra, Lipoplex-mediated transfection of mammalian cells occurs through the cholesterol-dependent clathrin-mediated pathway of endocytosis. ... IS Zuhorn,R Kalicharan,D Hoekstra - 《Journal of Biological Chemistry》 被引量: 455发表: 2002年 Clathrin-Independent Pathways of Endocytosis There are many...
Whereas rAAV2 is internalized via an HSPG- and clathrin-dependent pathway, HSPG-binding mutants used a clathrin- and caveolin-independent mechanism. Surprisingly, although this pathway was as efficient in mediating vector entry as the one used by rAAV2, successful cell transduction was hampered at...
Together our data not only provide additional evidence for the CME entry pathway for CCHFV, but also suggest for the first time that AP2 is likely to be a cargo adapter for CCHFV internalization. CCHFV infection is low pH-dependent After internalization within clathrin-coated vesicles, cargo ...
1B, treat- ment of cells by genistein (100 µg/ml) or herbimycin A (1 µM) had no influence on FcαR endocytosis, suggesting that tyrosine kinase activity was not required for FcαR endocytosis and further indicating that the endocytosis was mediated by a clathrin-dependent pathway. ...
The association of epsin with ubiquitinated cargo along the endocytic pathway is negatively regulated by its interaction with clathrin. Proc Natl Acad Sci USA 2005; 102: 2766–2771. Article CAS PubMed PubMed Central Google Scholar Antonin W, Holroyd C, Fasshauer D, Pabst S, Von Mollard GF...
摘要: These data demonstrate that ouabain stimulates a clathrin-dependent endocytosis pathway that translocates the Na/K-ATPase to intracellular compartments, thus suggesting a potential role of endocytosis in ouabain-induced signal transduction as well as proximal tubule sodium handling.关键词:...
Detailed analyses revealed that silibinin altered the formation of both clathrin-coated pits and vesicles in cells and caused abnormal uptake and trafficking of transferrin, a well-known cargo of the clathrin endocytic pathway. Silibinin also inhibited infection by other viruses that enter cells by ...