Beta-Lactamases of Ambler's Class A are the most commonly encountered mechanism of bacterial resistance to beta-lactam antibiotics. In the face of selective pressure arising from use of either newer cephalosporins or beta-lactam/beta-lactamase inhibitor combinations, mutations arose among Class A beta...
www.nature.com/scientificreports OPEN A new class A beta‑lactamase gene blaCAE‑1 coexists with blaAFM‑1 in a novel untypable plasmid in Comamonas aquatica Ying Li 1,2, Chengju Fang 1, Xu Wang 1, Qian Liu 3, Yichuan Qiu 1, Xiaoyi Dai 1...
Inhibition of class A and class C beta-lactamases by penems: crystallographic structures of a novel 1,4-thiazepine intermediate. A new β-lactamase inhibitor, a methylidene penem having a 5,6-dihydro-8H-imidazo[2,1-c][1,4]oxazine heterocyclic substituent at the C6 position with a Z c...
The pKa of Lys73 in both enzymes was computed to be above 10, in good agreement with recent experimental data on the TEM-1 beta-lactamase, but inconsistent with the proposal that Lys73 acts as the general base. Even when the closest titratable residue, Glu166, is mutated to a neutral ...
Analysis of a carbapenem-hydrolyzing class A beta-lactamase from Enterobacter cloacae and of its LysR-type regulatory protein Carbapenems such as imipenem are extended-spectrum β-lactam antibiotics, which are not hydrolyzed by the β-lactamases commonly found in Enterobacteriacea... T Naas,P Nordman...
Datz M, Joris B, Azab EA, Galleni M, Van Beeumen J, Frère JM, Martin HH. 1994. A common system controls the induction of very different genes. The class-A beta-lactamase of Proteus vulgaris and the enterobacterial class-C beta-lactamase. Eur J Biochem. 226(1):149-57. . :...
Serine-based β-lactamases of Class A, C and D all rely on a key water molecule to hydrolyze and inactivate β-lactam antibiotics. This process involves two conserved catalytic steps. In the first acylation step, the β-lactam antibiotic forms an acyl-en
SED-1, a class A β-lactamase from Citrobacter sedlakii , is a CTX-M-type extended-spectrum β-lactamase that has the ability to hydrolyze expanded-spectrum cephalosporins such as cefotaxime. SED-1 and a SED mutant in which Gly238 has been replaced by a cysteine, forming a disulfide bridge...
CTX-M-5, a novel cefotaxime-hydrolyzing beta-lactamase from an outbreak of Salmonella typhimurium in Latvia. At a children's hospital in Riga, Latvia, isolates identified as Salmonella typhimurium were found to be resistant to expanded-spectrum cephalosporins. Two... PA Bradford,Y Yang,D Sahm,...
Structural and computational characterization of the SHV-1 beta-lactamase-beta-lactamase inhibitor protein interface. β-Lactamase inhibitor protein (BLIP) binds a variety of class A β-lactamases with affinities ranging from micromolar to picomolar. Whereas the TEM-1 and ... Reynolds,A K. - 《Jo...