Cycloheximide (CHX) Chase Assay to Examine Protein Half-life” 的方法文章。 原文链接:www.bio-protocol.org/cn/bpdetail?id=4690 关键词:CHX 环己酰亚胺(CHX)追踪实验 蛋白质半衰期 蛋白质降解泛素泛素化 环己酰亚胺(cycloheximide,简称CHX)是一种常用的蛋白质合成抑制剂,广泛应用于生物研究中。它选择性地...
在近期的生物研究领域，苏州大学郑慧团队在Bio-Protocol杂志上揭示了环己酰亚胺（CHX）追踪实验的新应用，名为“Cycloheximide (CHX) Chase Assay to Examine Protein Half-life”。CHX作为一种经典的蛋白质合成抑制剂，通过阻断核糖体转位，成为了研究蛋白质降解、半衰期和转化率的有力工具。这篇方法论文验...
FigureS1.Cycloheximide(CHX)chaseassayand[35S]methioninepulse-chaseassayforthehalf-lifeofDrp1.A,293TcellsweretransfectedwithGFP-ParkinandGFP-Parkin(Δ..
the level of remaining Drp1 at different time points was quantified as the percentage of initial Drp1 level (0 hour of CHX treatment). C and D, the half-life of Drp1 in the presence of GFP-Parkin or GFP alone was analyzed by [35S] methionine pulse-chase assay, and the protein levels ...
Cycloheximide (CHX) chase assay and [35S] methionine pulse-chase assay for the half-life of Drp1. A, 293T cells were transfected with GFP-Parkin and GFP-Parkin (ΔR2) for 24 hours. Cells were then treated with CHX (100 μg/ml) for the indicated hours, and Western blotting was ...
Cycloheximide (CHX) chase assay and [35S] methionine pulse-chase assay for the half-life of Drp1. A, 293T cells were transfected with GFP-Parkin and GFP-Parkin (ΔR2) for 24 hours. Cells were then treated with CHX (100 μg/ml) for the indicated hours, and Western blotting was ...