Cycloheximide (CHX) Chase Assay to Examine Protein Half-life” 的方法文章。 原文链接:www.bio-protocol.org/cn/bpdetail?id=4690 关键词:CHX 环己酰亚胺(CHX)追踪实验 蛋白质半衰期 蛋白质降解泛素泛素化 环己酰亚胺(cycloheximide,简称CHX)是一种常用的蛋白质合成抑制剂,广泛应用于生物研究中。它选择性地...
在近期的生物研究领域，苏州大学郑慧团队在Bio-Protocol杂志上揭示了环己酰亚胺（CHX）追踪实验的新应用，名为“Cycloheximide (CHX) Chase Assay to Examine Protein Half-life”。CHX作为一种经典的蛋白质合成抑制剂，通过阻断核糖体转位，成为了研究蛋白质降解、半衰期和转化率的有力工具。这篇方法论文验...
the level of remaining Drp1 at different time points was quantified as the percentage of initial Drp1 level (0 hour of CHX treatment). C and D, the half-life of Drp1 in the presence of GFP-Parkin or GFP alone was analyzed by [35S] methionine pulse-chase assay, and the protein levels ...
Cumulative fatigue damage and life prediction theories--a survey of the state of the art for homogeneous materials 热度: 100 Simple Secrets of the Best Half of Life 热度: FigureS1.Cycloheximide(CHX)chaseassayand[ 35 S]methioninepulse-chaseassayfor ...
Cycloheximide (CHX) chase assay and [35S] methionine pulse-chase assay for the half-life of Drp1. A, 293T cells were transfected with GFP-Parkin and GFP-Parkin (ΔR2) for 24 hours. Cells were then treated with CHX (100 μg/ml) for the indicated hours, and Western blotting was ...
原文链接:Cycloheximide (CHX) Chase Assay to Examine Protein Half-life 环己酰亚胺(CHX)追踪测定法检测蛋白质半衰期 ● Polysome Profiling in Adult Mouse Testes 成年小鼠睾丸中的多聚体分析 作者:Jun-Yan Kang、Ai Zhong、Ze Wen、Xinghai Yu、Yu Zhou、Mo-Fang Liu ...
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| cellresearchcorp | cellsciences | cellscript | cellsignal | celltechnology | Celltreat Scientific Products | cellular technology ltd | cellutions biosystems | cellworks | celsis | cemines | cerilliant | ch3 biosystems | chantest | charles river laboratories | Chase Instruments | Chemetrics Inc...