Cycloheximide (CHX) Chase Assay to Examine Protein Half-life” 的方法文章。 原文链接:www.bio-protocol.org/cn/bpdetail?id=4690 关键词:CHX 环己酰亚胺(CHX)追踪实验 蛋白质半衰期 蛋白质降解泛素泛素化 环己酰亚胺(cycloheximide,简称CHX)是一种常用的蛋白质合成抑制剂,广泛应用于生物研究中。它选择性地...
Once protein synthesis is inhibited by CHX, the level of intracellular proteins decreases by degradation through the proteasome or lysosome system. Thus, the CHX chase assay is widely recognized and used to observe intracellular protein degradation and to determine the half-life of a given protein ...
在近期的生物研究领域，苏州大学郑慧团队在Bio-Protocol杂志上揭示了环己酰亚胺（CHX）追踪实验的新应用，名为“Cycloheximide (CHX) Chase Assay to Examine Protein Half-life”。CHX作为一种经典的蛋白质合成抑制剂，通过阻断核糖体转位，成为了研究蛋白质降解、半衰期和转化率的有力工具。这篇方法论文验...
● Cycloheximide (CHX) Chase Assay to Examine Protein Half-life 环己酰亚胺(CHX)追踪测定法检测蛋白质半衰期 作者:Ying 、Qian Du、Hong-Guang Zhang、Yibo Zuo、Hui Zheng、Yukang Yuan 原文链接:Cycloheximide (CHX) Chase Assay to Examine Protein Half-life 环己酰亚胺(CHX)追踪测定法检测蛋白质半衰期 ● ...
half-lifeofDrp1inthepresenceofGFP-ParkinorGFPalonewasanalyzedby[ 35 S] methioninepulse-chaseassay,andtheproteinlevelswerequantified. FigureS2.InvitroubiquitinationofDrp1byMARCH5.InvitrotranslatedDrp1wasincubated withpurifiedubiquitin,E1,E2(UbcH7),variousParkinproteinsandimmunoprecipitated ...
the level of remaining Drp1 at different time points was quantified as the percentage of initial Drp1 level (0 hour of CHX treatment). C and D, the half-life of Drp1 in the presence of GFP-Parkin or GFP alone was analyzed by [35S] methionine pulse-chase assay, and the protein levels ...
Cycloheximide (CHX) chase assay and [35S] methionine pulse-chase assay for the half-life of Drp1. A, 293T cells were transfected with GFP-Parkin and GFP-Parkin (ΔR2) for 24 hours. Cells were then treated with CHX (100 μg/ml) for the indicated hours, and Western blotting was ...