chromosome conformation capture称之为染色质构象捕获,简写为3C, 是一种生物化学手段,可以通过实验手段来研究空间结构上相近的染色质,本文简单介绍下3C的实验过程和原理。 实验流程如下所示 可以分成以下4个步骤 crosslink chromatin, 染色质交联。通过甲醛固定细胞,可以在空间结构相邻的染色质片段之间产生共价氢键 digest...
ChIA-PET,HiChIP,PLAC-seq 仅能捕获某一种蛋白介导的染色质间的相互作用; Capture Hi-C技术通过特异性探针,捕获探针所在区域(如启动子区)染色质间相互作用; DNase Hi-C虽然可以富集开放染色质之间的相互作用,但是由于DNase I整合效率非常高,高度片段化的染色质之间自连或随机连接,减少了真实相互作用的捕获比例. B...
在过去10年中,染色体构象捕获(chromosome conformation capture 3C)技术及其该技术的拓展技术(4C,5C,Hi-C,ChIA-PET),使人们能够以超强的分辨率和高通量测序分析细胞核内的三维立体结构 3C技术:走向三维(3D)基因组学的基础 Fig1 1.交联:用甲醛交联染色质,固定蛋白与DNA,使染色质保持三维结构。 2酶切:再用一种限...
MIT 分子生物学 part1 9.3 解旋酶上样和染色质免疫沉淀(ChIP) - 哔哩哔哩 (bilibili.com) MIT 分子生物学 part1 8.6 模板结合分析实验 - 哔哩哔哩 (bilibili.com) https://www.jianshu.com/p/70cbd26294d3 http://www.biomarker.com.cn/archives/13520...
Visithttp://.epigenesys.euforotherepigeneticsandsystemsbiologyprotocols Page1of9 QuantitativeChromosomeConformation Capture(3C-qPCR) VuthyEa 1 ,ThierryForné 1 andFranckCourt 1,2 1 InstitutdeGénétiqueMoléculaire,UMR5535CNRSUniversityofMontpellier,France 2 Presentaddress:InsermUMR1103,CNRSUMR6293,F-63001...
Chromosome conformation capture (3C) assays are used to map chromatin interactions genome-wide. Chromatin interaction maps provide insights into the spatial organization of chromosomes and the mechanisms by which they fold. Hi-C and Micro-C are widely used 3C protocols that differ in key experimental...
Chromosome conformation capture (3C) technology is a pioneering methodology that allows in vivo genomic organization to be explored at a scale encompassing a few tens to a few hundred kilobase-pairs. Understanding the folding of the genome at this scale is particularly important in mammals where di...
GenomeDISCO: A concordance score for chromosome conformation capture experiments using random walks on contact map graphs. The three-dimensional organization of chromatin plays a critical role in gene regulation and disease. High-throughput chromosome conformation capture exper... Oana,Ursu,Nathan,... ...
The chromosome conformation capture (3C) method has been invaluable in studying chromatin interactions in a population of cells at a resolution surpassing ... MV Rudan,S Hadjur,T Sexton - 《Methods Mol Biol》 被引量: 7发表: 2016年 Discovering hotspots in functional genomic data superposed on ...