Chromatin is a substance made up of DNA or RNA and proteins, such as histones. It condenses during cell division (mitosis or meiosis) and becomes a chromosome. Chromatins are“unwound” condensed structures whereas chromosomes are highly packaged and more condensed than chromatins. Thus, chromosom...
The relationship between chromatin organization and gene regulation remains unclear. While disruption of chromatin domains and domain boundaries can lead to misexpression of developmental genes, acute depletion of regulators of genome organization has a
e Transcription factor motif enrichments for the isolated analysis of punch F5, indicating that cluster 1 (n = 44 cells) is made up of glutamatergic neurons and cluster 2 (n = 45 cells) is made up of other cell types. Center line represents median, lower, and upper hinges ...
The iconic DNA double helix is only the first level of organization of the hereditary material in a cell. Beyond the helix, DNA wraps around barrel-shaped nucleosomes made of histone proteins and forms a higher order fiber, which then folds onto itself to form chromatin loops. Neighboring ...
Ninety percent of the accessible regions locate to intergenic regions containing one or two putative promoter regions (Figure 1C). Within these regions, the majority of peaks locate up to 2 kb upstream of the ATG and, when a transcription start site (TSS) is known (Kensche et al., 2016)...
PCR primers used for real time PCR amplification of DNA isolated by the ChIP process were as follows: LTR-forward, 5′-TACAAGGGACTTTCCGCTGG-3′, and LTR-reverse, 5′-AGCTTTATTGAGGCTTAAGC-3′ for the Nucleosome-1 (Nuc-1) region of the LTR; LTRup-forward, 5′-CCAAAGAAGACAAGATATCCT...
The meaning of CHROMATIN is a complex chiefly of DNA and histone in eukaryotic cells that is usually dispersed in the interphase nucleus and condensed into chromosomes in mitosis and meiosis and in which the nucleosome makes up its repeating subunits. Ho
(see Fig.2). Our simulations not only reproduce the initial slope, which would be trivial, but also apparently reproduces the more complex experimental diffusive motion of interphase chromosomes in the nucleus. This means that by multiplying the elapsed time in each simulation trajectory by\up...
We also note the presence of small GFP-positive granules in all these muscle cells (Figure 1d). We do not know the identity of these punctate structures, but we do not think they are mitochondria, as there are far more mitochondria that take up targeted GFP in C. elegans body wall ...
Phenotypes of transgenic Arabidopsis plants with different tagging constructs. (A) Wild-type Arabidopsis at the rosette stage, (D) at the inflorescence stage, and (G) a close-up of a flower. (B) Line withAG-eGFPfusion construct showing anAGoverexpression phenotype (pARC276). (C) Line with...