Chromatin is a substance made up of DNA or RNA and proteins, such as histones. It condenses during cell division (mitosis or meiosis) and becomes a chromosome. Chromatins are“unwound” condensed structures whereas chromosomes are highly packaged and more condensed than chromatins. Thus, chromosom...
The relationship between chromatin organization and gene regulation remains unclear. While disruption of chromatin domains and domain boundaries can lead to misexpression of developmental genes, acute depletion of regulators of genome organization has a
Follow-up examination of 15 unselected children with aneuploid sex chromosome abnormalities has been made till between the age of 2 1/2 and 5 years. The mental development of the 15 children was in all cases within the normal range, but ... J Nielson,I Sillesen - 《Human Genetics》 被引...
The iconic DNA double helix is only the first level of organization of the hereditary material in a cell. Beyond the helix, DNA wraps around barrel-shaped nucleosomes made of histone proteins and forms a higher order fiber, which then folds onto itself to form chromatin loops. Neighboring ...
HCT116 cells have large ungapped H3K9me3 (B4) domains up to several megabases in length (Fig. 3e and Extended Data Fig. 6a,b). Taking the largest domains ranked by size for each of the other cell types, we observe that K562 and fibroblasts (HFFc6, IMR90) also exhibit large domains....
alternata. More importantly, theldl4mutants are partially defective in JA signaling, but hyperactive in ethylene signaling (Singh et al.2019). Recently, theldl1 ldl2double mutant was shown to exhibit resistance toPstDC3000, which may be caused, in part, by H3K4me3-dependent upregulation ofWRKY...
RNA-seq experiments revealed an A-fat and GF-fat selective gene expression signature, with 126 genes upregulated in abdominal preadipocytes and 90 genes upregulated in GF cells. ATAC-seq identified almost 10-times more A-specific chromatin-accessible regions. Using a combined analysis of ATAC-seq...
3C). Transcripts of Scp3 were first detectable on day 6, upregulated to the highest levels on day 10, and then decreased sharply on day 14, consistent with progression to the diplotene stage. Transcripts of haploid spermatid markers, such as Prm1, Tnp1, Haprin, and Acr, were first ...
We also note the presence of small GFP-positive granules in all these muscle cells (Figure 1d). We do not know the identity of these punctate structures, but we do not think they are mitochondria, as there are far more mitochondria that take up targeted GFP in C. elegans body wall ...
For setting up the repression, cells were peletted down in mid-log phase and shifted to pre- warmed 0.15X YEP medium without any carbon source and incubated for 4 hours [22]. Tiling Array Design A high density tiling array (244 K Chip) of Agilent platform containing 60-mer probes was ...