Poised enhancers (PEs) represent a genetically distinct set of distal regulatory elements that control the expression of major developmental genes. Before becoming activated in differentiating cells, PEs are already bookmarked in pluripotent cells with unique chromatin and topological features that could co...
Chromatin is the genetic material of eukaryotes. The core of a genomic chromatin fiber is an array of nucleosomes. Chromatin condenses the chromosomal DNA molecule into a globular territory in the nucleus. At the same time, chromatin is the substrate for functional processes such as transcription....
Hint:Chromosomes are made up of chromatin. Chromatin made up of the threads of DNA forms the structure of chromosomes. The DNA gets condensed to form chromosomes. Genes are the segments of DNA. The code for proteins. This means chromosomes are made up of DNA which contain...
The eukaryotic genome is tightly packed in the form of chromatin, which consists of DNA and histone-formed nucleosomes as basic subunits. Chromatin compaction restricts the access of transcription factors tocis-regulatory elements such as promoters and enhancers, and thus affecting gene transcription3,...
Similar to spatial protein expression analysis, Llorens-Bobadilla et al. have recently adapted their ST approach to measure open chromatin information using spatial ATAC-Seq [48]. Another interesting development has been the combination of spatial transcriptomics and expansion microscopy called Ex-ST [...
Altering the biochemical makeup of chromatin by the incorporation of histone variants during development represents a key mechanism in regulating gene expression. The histone variant H2A.B, H2A.B.3 in mice, appeared late in evolution and is most highly expressed in the testis. In the mouse, it...
downstream receptor IL-11RA and enrichment of signal pathway to clarify the potential autocrine mechanism of IL-11.Next, chromatin immunoprecipitation coupled with high-throughput sequencing (ChIP-seq) was performed to detect the nuclear localization and DNA-binding patterns of phosphorylated STAT4 (p...
While there are in vitro and cell-based high-throughput techniques, such as chromatin immunoprecipitation followed by sequencing42, it may be excessive depending on the final application. One advantage of the nanopore system is a few sequences of interest can be tested with minimal sample amounts...
In the proter, parental adoral membranelles are mostly retained, with only those in the posterior end renewedin situ(Figs.4c,d,5c–f). The UMA is formed from the dedifferentiation of the parental undulating membranes (Figs.4b–d,5b,c). In subsequent stages, the development of the UMA fol...
The complex language of chromatin regulation during transcription Nature, 447 (2007), pp. 407-412 Google Scholar Berthiaume et al., 2011 F. Berthiaume, T.J. Maguire, M.L. Yarmush Tissue engineering and regenerative medicine: history, progress, and challenges Annu. Rev. Chem. Biomol. Eng., ...