Recombinant CHO cell line development (CLD) has been a crucial step for therapeutic protein production platforms; however, this step remains time-consuming and costly. With the emergence of multiomics data sets of CHO cells and genome editing technology such as CRISPR/Cas9, site-specific ...
2) Cell line development. Recent developments in omics technologies have been essential for the development of rational methods of constructing a cell line. 3) Cell engineering for post-translational steps. Improvement of secretion, folding and glycosylaiton is an important key issue for mammalian ...
4 Bielser JM, et al. Perfusion Mammalian Cell Culture for Recombinant Protein Manufacturing — A Critical Review. Biotechnol. Adv. 36(4) 2018: 1328–1340; https://doi.org/10.1016/j.biotechadv.2018.04.011. 5 Zeh N, et al. Cell Line ...
[3] Tihanyi B, Nyitray L. Recent advances in CHO cell line development for recombinant protein production. Drug Discov Today Technol. 2020 Dec;38:25-34. [4] Wurm MJ, Wurm FM. Naming CHO cells for bio-manufacturing: Genome plasticity and variant phenoty...
4、also discussedKey words: monoclonal antibody; CHO cell; cell line development; cell culture process20 世纪 80 年代初,基因重组技术开始应用 于动物 细胞。1984 年,Genentech 公司首次实现 重组中国仓鼠卵巢 ( Chinese hamster ovary,CHO) 细胞表达组织型纤溶酶原激活剂( tissue type plas- minogen activato...
GenScript establishes the TurboCHO™ stable cell pool/cell line expression platforms with ECACC-licensed CHO-K1 cells. Coupling with an Ai-powered patented GenSmart codon optimization system and continuously optimized fed-batch cultural process, GenScr
In this review article, we focus on current strategies and achievements in cell line development, mainly in vector engineering and cell engineering, for high and stable protein production in rCHO cells. The approaches that manipulate various DNA elements for gene targeting by site-specific ...
Key words: CHO cells; CRISPR/Cas9; gene editing; site-specific synchronization; cell line construction CHO 细胞作为目前生物制药工业领域生产重组 蛋白的"主力军",拥有诸多优势,如生长快速,易 于适应无血清悬浮培养,易于放大,生产工艺成熟, 易转染以及安全性高等[1-3].尽管 CHO 细胞获得了 广泛的应用,...
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BASAL CHO MX growth medium provides optimal balance of amino acids & other nutrients to ensure adequate cell growth & maximum productivity of your process