been optimized and standardized, the cell-cycle time of all cell types used in the test should be determined under typical negative control conditions in the laboratory; values obtained should be consistent with the published values. It is useful to establish representativekaryotypesof cell lines on...
(TALENs), and clustered regularly interspaced short palindromic repeats (CRISPR)-associated (Cas) RNA guided nucleases are technologies readily available to induce targeted insertion/deletion (indel) mutations or precise sequence changes in a broad range of organisms and cell types (reviewed in Ref. ...
Response of a CHO production cell line to different types of hydrodynamic shear stress present in stirred and sparged bioreactorsIn this work, the response and adaption of CHO cells to hydrodynamic stress in laboratory scale biore-actors originating from agitation, sparging and their combination is ...
Spontaneously transformed (tumorigenic) Chinese hamster cells (line CHO) do not exhibit picolinic acid-sensitive G 1 and G 2 cell cycle arrest points observed in normal and virus-transformed cells. Rather, picolinic acid arrests CHO cells in S phase only and produces culture growth behaviour simila...
Cell line dependencies and additional component considerations Frequently, cell line specific component dependencies, like insulin and other growth factors, are missed when evaluating media. These are components that are needed for your cell line to thrive (growth, productivity) and if they are not pr...
Cytoskeleton, endoplasmic reticulum and nucleus alterations in CHO-K1 cell line after Crotalus durissus terrificus (South American rattlesnake) venom treat... Snake venoms are toxic to a variety of cell types. However, the intracellular damages and the cell death fate induced by venom are unclear....
-arrested cho cell line [ 30 ]. we also found altered expression of the ins2 gene in the cl47[1] cell line; this gene is not commonly expressed in non-pancreatic β cells. it is known that all cells contain an insulin gene, but its expression can vary in different cell types. the ...
Upon nutrient deprivation, Chinese hamster ovary (CHO) cells are subjected to two types of programmed cell death, apoptosis and autophagy. CHO cell enginee... OH Sun,GM Lee - 《Journal of Biotechnology》 被引量: 52发表: 2009年 Engineering Chinese hamster ovary (CHO) cells to achieve an inv...
皮肤肥大细胞Many types of cells包装:5 × 105方(1ml)地塞米1酸钠Dexamethasone Phosphate质量规格:>98%,USP CHO细胞ZR-75-1人癌细胞 Human breast cancer cell line ZR-75-1 RPMI-1640(GIBCO)+10%FBS盐酸乙哌立(标准品)质量规格:含量测定Eperisone hydrochloride ...
皮肤肥大细胞Many types of cells包装:5×105方(1ml)地塞米1酸钠Dexamethasone Phosphate质量规格:>98%,USP CHO细胞ZR-75-1人癌细胞Human breast cancer cell line ZR-75-1 RPMI-1640(GIBCO)+10%FBS盐酸乙哌立(标准品)质量规格:含量测定Eperisone hydrochloride ...