Protocol 1: Preparation of Plasmid DNA by Alkaline Lysis with SDS: Minipreps Protocol 2: Preparation of Plasmid DNA by Alkaline Lysis with SDS: Maxipreps Protocol 3: Isolating DNA from Gram-Negative Bacteria (e.g.,E. coli) Protocol 4: Precipitation of DNA with Ethanol ...
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Here, we have outlined a chromatin immunoprecipitation (ChIP) protocol for use in whole Caenorhabditis elegans extracts. We discuss optimization of the procedure, including growth and harvesting of the worms, formaldehyde fixation, TF immunoprecipitation and analysis of bound sequences through real-time ...
Samples then underwent a 20 Ǎ/ qRT-PCR protocol using the C1000 TouchTM Ther- mal Cycler (BioRad) at 95°C for 10 min, 95°C for 15 s and 60°C for 60 s, before being repeated for 39 more cycles and held at 4°C. All qRT-PCR data was analysed using CFX ManagerTM (Bio...
A universal protocol for grafting PCR primers onto glass, PDMS, COP, COC, and PP is developed and evaluated by solid-phase PCR (SP-PCR). Primers are immobilized in a PCR compatible way featuring spots with high homogeneity and integrity. Furthermore, we show a protocol for binding a PCR...
modification. An example of this would include measurement of the amount of histone H3 acetylation associated with a specific gene promoter region under various conditions that might alter expression of the gene. Histones are not the only proteins that can be studied using this technique. Much of ...
In this chapter, a protocol called on-chip polymerase chain reaction (PCR) is presented for the (DNA) microarray-based detection of bacterial target sequen... G Mitterer,WM Schmidt - 《Methods in Molecular Biology》 被引量: 27发表: 2006年 Promoter hypermethylation-induced transcriptional down-reg...