Herein, we propose the use of a microfluidic-based method to rapidly detect drug-resistant gene mutations using Kompetitive Allele-Specific PCR (KASP).Methods: A total of 300 clinical samples were collected, and DNA extraction was performed using the "isoChip" Mycobacterium detection k...
This protocol is developed and tested for E. coli. It should work the same way for other bacteria but that remains to be proven. Published protocols also exist for other bacterial species, including Bacillus subtilis, Caulobacter crescentus and Mycobacterium tuberculosis. Because E. coli can be ...
RT-qPCR assay of the 5th WHO International Standard for HCV spanning the range of 15–10000 IU/mL in EDTA-plasma was performed on the µTASWako g1 Analyzer. Figure1ashows the overlay of electropherograms of the PCR cycles for 1000 IU/mL of HCV RNA. The graph depicts the growth of t...
This review covers a developmental progression on early to modern taxonomy at cellular level following the advent of electron microscopy and the advancement in deoxyribonucleic acid (DNA) extraction for expatiation of biological classification at DNA level. Here, we discuss the fundamental values of co...
and PCR are generally not suitable for rapid, in-field biodetection6,13. These sensors usually perform only single point measurements and are incapable of continuously monitoring molecular analytes. The real-time monitoring of physiological species in body fluids has medical importance. For example, co...
当加载的患者样本,微流器件依次进行的片上的细胞裂解,RNA的纯化和浓缩步骤的固相萃取(SPE),反转录(RT)和聚合酶链反应(PCR),在RT-PCR室内,分别。终点检测使用片外生物分析仪(安捷伦科技,圣克拉拉,CA)。用于外围设备,我们使用一个单一的注射器泵进行驱动的试剂和样本,而两个薄膜加热器用作热源的RT和PCR室。该...
Ramalingam N, Long-Qing C, Yang XH, Deng LQ, Wang QH, Huat EYP, Neo CH, Gong HQ (2009) A surface-directed microfluidic scheme for parallel nanoliter pcr array suitable for point-of-care testing. ICNMM Pts a-B:1007 Suriano R, Hume J, Cereda M, De Fazio M, Bianchessi M, Levi ...
To implement the protocol on the In-check platform, the primers were tested in combination and two multiplex PCR were developed; the optimum annealing temperature for the primer pairs was identified as 61 ◦C (Table 2). The sensitivity comparison between the conventional real-time PCR and ...