通过ChIP-qPCR分析转录因子结合位点的富集程度,为转录因子结合位点的功能研究提供实验依据。DNA免疫沉淀检测ChIP qPCR ChIP-seq实验虽然是一种强大的研究蛋白质与DNA相互作用的技术,但也存在一些缺点。染色质免疫共沉淀检测ChIP-RT-PCR检测 ChIP-seq与ChIP-qPCR在实验原理和应用方面存在一些相同点。首先,它们的实验原理都...
定量RT-PCR Fold change 法 适用于比较两个不同标本(如标本A与标本B)的基因表达差异 ChIP-定量PCR与定量RT-PCR ChIP-定量PCR 1 定量RT-PCR 2 ChIP概念 染色质免疫沉淀(Chromatin immunoprecipitation,ChIP)是研究体内蛋白质与DNA相互作用的一种技术。它利用抗原抗体反应的特异性,可以真实地反映体内蛋白因子与基因...
请问CHIP-RTPCR中β-actin在结果处理中的作用是?我看数据分析里没有提到它的作用,望大神指教!谢谢! 分子生物 EMSA/ChIP
请问IgG可以稀释一下吗，降低非特异结合。还有如何用β-actin平衡数据呀
Go to NCBI primer design[2]( attr(href) ) Enter your sequence in the first box. PCR product size should be set to 70-150bp. Make sure to select the proper species in the “organism” section. Click ‘Get Primers’. Choose a primer pair that is towards the middle region, if availab...
An apparatus (100) for performing thermal cycles has a frame (131-134, 137, 147) enclosing a thermal chamber (110) laterally delimited by delimitation walls (103, 154) and is configured to detect, in use, a feature of the light radiation emitted.ENZO FONTANAANTONINO SCUDERI...
An innovative polymer lab-on-a-chip (LOC) for reverse transcription (RT)-polymerase chain reaction (PCR) has been designed, fabricated, and characterized for point-of-care testing (POCT) clinical diagnostics. In addition, a portable analyzer that consists of a non-contact infrared (IR) based ...
Norgen Biotek 细胞质和细胞核RNA提取kit:一个样品,同时分别提取细胞质RNA与细胞核RNA,提取的RNA可直接用于下游应用:RT-PCR, qRT-PCR, RNA-Seq, Northern blotting, chip - 艾美捷科技于20240108发布在抖音,已经收获了347.4万个喜欢,来抖音,记录美好生活!
Rapid detection for primary screening of influenza A virus: microfluidic RT-PCR chip and electrochemical DNA sensor. Keiichiro Yamanaka,Masato Saito,Kenji Kondoh, et al. The Analyst . 2011Yamanaka K,Saito M,Kondoh K,et al.Rapid detection forprimary screening of influenza A virus:microfluidic RT-...
百度试题 题目下面那些方法能检测某一特定的mRNA水平?() A.RT-PCRB.Real-Time PCRC.ChIPD.Northern blot相关知识点: 试题来源: 解析 A,B,D 反馈 收藏