The in vitro cell migration and invasion assays were performed by using a modified Boyden chamber inserted with polyethylene terephthalate filter membrane containing 8-μm pores in 24-well plates (Millipore, Billerica, MA). For cell invasion assays, the filter membranes were coated with Matrigel (...
Purified water was prepared by the Milli-Q system (Billerica, USA; electrical resistivity: 18.2 MΩ·cm) and then filtered through 0.22 μm microfiltration membrane before use. BHGZ decoction was provided by Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences (Beijing, ...
Ultrapure water was prepared with a Milli-Q water purification system (Millipore, 7 Billerica, MA, USA). HPLC grade acetonitrile and methanol were obtained from Merck (Darmstadt, Germany). Ammonium acetate and fetal bovine serum were purchased from Thermo Fisher Scientific (Rockford, USA). Formic...
Deionized water was prepared by the Milli-Q system (Millipore Corporation, Billerica, MA). All other chemicals were purchased from Sigma-Aldrich (St. Louis, MO, USA) or Thermo Fisher Scientific (Waltham, MA, USA). 2.2. Preparation of sanguisorbin extract solution The powder of sanguisorbin ...
(Millipore, Billerica, MA, USA) according to the manufacturer’s protocol. PC12 cells were lysed in complete RIP lysis buffer, and the cell extract was incubated with protein A/G agarose beads conjugated with antibody Bsg or control IgG for 2 h at 4 °C. Beads were washed and incubated ...
Solid-state 13C-NMR spectra were obtained with a Bruker Avance AV-400 (Bruker Corp., Billerica, MA) spectrometer at 100.62 MHz, with a repetition time of 110.7 ms, and an echo time of 4.5 ms. Size exclusion chromatography was accomplished by use of an Aglient 1200 instrument with a YMC...
Putative colonies of each morphotype were selected for blaNDM gene detection, with the positive isolates then identified by matrix-assisted laser desorption/ionization-time-of-flight mass spectrometry (Bruker, Billerica, MA, USA) and 16S rRNA gene sequence-based analyses. The total DNA of ...
Mass spectrometry data suggests that the 70-kDa protein belongs to the HSP 70 family. SCIT inhibited the inflammatory response in the long term and a 70-kDa protein potentially belonging to the HSP 70 family plays a significant role in Chinese birch pollen-induced mice model....
In this work, an approach was developed by integrating activity index (AI), liquid chromatography – mass spectrometry (LC-MS), and nuclear magnetic resonance (NMR) to rapidly predict and identify the potential active constituents from TCM. This approach was used to discover and identify the ...
Water was purified with a Milli-Q water purification apparatus (Millipore, Billerica, MA, USA). The immortalized hepatic cell line HepaRG was obtained from the Type Culture Collection of the Chinese Academy of Sciences (Shanghai, China). The following reagents were also used in this study: ...