Equal amounts of protein were separated using 8–12% sodium dodecyl sulfate-polyacrylamide (SDS-PAGE) and transferred to polyvinylidene difluoride (PVDF) membranes (Millipore, Billerica, MA, USA). Next, the membranes were blocked with 5% skim milk for 2 h at room temperature and incubated over...
Deionized water was prepared by the Milli-Q system (Millipore Corporation, Billerica, MA). All other chemicals were purchased from Sigma-Aldrich (St. Louis, MO, USA) or Thermo Fisher Scientific (Waltham, MA, USA). 2.2. Preparation of sanguisorbin extract solution The powder of sanguisorbin ...
The in vitro cell migration and invasion assays were performed by using a modified Boyden chamber inserted with polyethylene terephthalate filter membrane containing 8-μm pores in 24-well plates (Millipore, Billerica, MA). For cell invasion assays, the filter membranes were coated with Matrigel (...
The PC12 cells were pre-treated with nerve growth factor 7S (Millipore, Billerica, MA, USA) to obtain neuron-like cells [25]. Cell viability measurements by MTT assay Cell viability was assessed by using a 3-(4,5-dimethythiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay. ...