Equal amounts of protein were separated using 8–12% sodium dodecyl sulfate-polyacrylamide (SDS-PAGE) and transferred to polyvinylidene difluoride (PVDF) membranes (Millipore, Billerica, MA, USA). Next, the membranes were blocked with 5% skim milk for 2 h at room temperature and incubated over...
Signaling, delivery and age as emerging issues in the benefit/risk ratio outcome of tPA For treatment of CNS ischemic disorders. J Neurochem. 2010;113:303–12. Article CAS PubMed PubMed Central Google Scholar Olmez I, Ozyurt H. Reactive oxygen species and ischemic cerebrovascular disease. ...
Caspase-3 activity was measured with caspase-3 Colorimetric Assay Kit (Millipore, Billerica, MA) as previously described56. Briefly, at 3 hours after MI/R, hearts were excised and homogenized and 100 μg of total protein from heart tissues was loaded onto each well of a 96-well plate...
Deionized water was prepared by the Milli-Q system (Millipore Corporation, Billerica, MA). All other chemicals were purchased from Sigma-Aldrich (St. Louis, MO, USA) or Thermo Fisher Scientific (Waltham, MA, USA). 2.2. Preparation of sanguisorbin extract solution The powder of sanguisorbin ...
The in vitro cell migration and invasion assays were performed by using a modified Boyden chamber inserted with polyethylene terephthalate filter membrane containing 8-μm pores in 24-well plates (Millipore, Billerica, MA). For cell invasion assays, the filter membranes were coated with Matrigel (...
This raised the possibility that increasing copper delivery to PAM could increase peptide amidation. However, the nanomolar levels of copper in DMEM/F12 (5 nM) appear to be adequate; raising the copper concentration in the medium five-fold had no effect on the extent of amidation. Higher ...
(Millipore, Billerica, MA, USA). The membrane was incubated in blocking buffer (5% skim milk (Fluka, Buchs, Switzerland) in Tris-Buffered Saline (TBS) plus 0.5% (v/v) Tween 20). The blot was incubated for 1 h 30 min at room temperature (RT) with either mouse anti-human EpCAM...