View the video: Monoclonal Antibody Analysis Update: Better Separations of MAb variantsProven Charge Variant Workflow at global biopharma CRO Speed and accuracy are key criteria for laboratories performing charge variant profiling. Especially in contract research...
View the video: Monoclonal Antibody Analysis Update: Better Separations of MAb variantsProven Charge Variant Workflow at global biopharma CRO Speed and accuracy are key criteria for laboratories performing charge variant profiling. Especially in contract research or...
Thermo Fisher Scientific, Thermo Scientific Application Note 20784, A Novel pH Gradient Separation Platform for Monoclonal Antibody (mAb) Charge Variant Analysis, AN20784_E, Sunnyvale, CA, 2013Lin, S.; Baek, J.; Rao, S.; Agroskin, Y.; Pohl, C.; Decrop, W.; Mi...
This pH gradientmethod serves as a platform method for the mAb charge variant analysis, covering the pH range from 5.6 to 10.2. Most of the therapeutic mAbs have pI values fallingwithin this pH range. Rituxiamab (Figure 1a),... TF Scientific,Sunnyvale,S Lin,... 被引量: 0发表: 2015年...
As shown in Figure 3C, the BioResolve SCX mAb Column was able to perform with comparable results in terms of charge variant profile and reproducibility (%RSD < 1.0) when using a salt gradient, demonstrating the columns ability to perform robustly with more ...
This pH gradientmethod serves as a platform method for the mAb charge variant analysis, covering the pH range from 5.6 to 10.2. Most of the therapeutic mAbs have pI values fallingwithin this pH range. Rituxiamab (Figure 1a),trastuzumab (Figure 3a), adalimumab (Figure 5a), andbevacizumab (...
The analysis ensures product consistency with no unwanted changes to the protein. With increasing numbers of new mAb drug products emerging in the market, the need for a robust charge variant analysis has intensified. The charge variant profiles often display partially resolved peaks on shoulders of...
In the case of IEX lysine charge variant analysis of infliximab, the measure of importance is the level of each charge variant present in solution. Relative area counts of the three lysine charge variant peaks are given in Table II...
Carboxypeptidase B digestion confirmed that the major basic variant peaks in biosimilar correspond to lysine variants. The Agilent 1260 Infinity Bio-inert Quaternary LC with Bio MAb PEEK columns and reproducible method make this solution particularly suitable for the QA/QC analysis of mAbs for the ...
the application of dual pH/salt gradients for the optimization of size and charge variant separation of mAbs is described.A stoichiometric displacement model is used to characterize and to predict protein elution in a combination of both salt and pH gradients(parallel-antiparallel,increasing-decreasing...