Thus, these results provided meaningful information for the comparative evaluation of charge-related heterogeneity of mAbs, and suggested that charge variants of IgGs do not affect the in vitro potency, FcRn binding affinity, or the PK properties in rats. 展开 ...
Charge variants in IgG1: isolation, characterization, in vitro binding properties and pharmacokinetics in rats. mAbs . 2010; 2 (6):613–624. doi: 10.4161/mabs.2.6.13333. [ Cross Ref ]Khawli LA, Goswami S, Hutchinson R, Kwong ZW, Yang J, Wang X, . Charge variants in IgG1: Isolation...
We developed our next generation cation exchange column to extend column lifetime and method robustness - the Thermo Scientific MAbPac SCX-10 LC Column is for high-resolution, high efficiency analysis of monoclonal antibodies (mAbs) and associated variants....
We developed our next generation cation exchange column to extend column lifetime and method robustness - the Thermo Scientific MAbPac SCX-10 LC Column is for high-resolution, high efficiency analysis of monoclonal antibodies (mAbs) and associated variants. The...
This application note describes the use of the Agilent Bio MAb weakcation-exchange column in measuring charge variants of a monoclonal antibody,and the subsequent characterization of these variants after peak collection. Analysis was achieved by reversed-phase liquid chromatography and mass spectrometry.收...
Most of the therapeutic mAbs have pI values fallingwithin this pH range. Rituxiamab (Figure 1a),... TF Scientific,Sunnyvale,S Lin,... 被引量: 0发表: 2015年 Development and Validation of Salt Gradient CEX Chromatography Method for Charge Variants Separation and Quantitative Analysis of the ...
mAbs are highly complex proteins that present a wide range of microheterogeneity that requires multiple analytical methods for full structure assessment an... M Biacchi,R Bhajun,N Sa?D,... - 《Electrophoresis》 被引量: 44发表: 2015年 Conformation and Dynamics of Interchain Cysteine-Linked Antibo...
Report mAbs 5:1, 114–125; January/February 2013; © 2013 Landes Bioscience Conformational characterization of the charge variants of a human IgG1 monoclonal antibody using H/D exchange mass spectrometry Liangjie Tang,* Shanmuuga Sundaram, Jingming Zhang, Ping Carlson, Alice Matathia, Babita ...
Ion Exchange Chromatography (IEX) is a valuable technique given its ability to resolve product/process related charge variants as part of the development and manufacturing process of biopharmaceuticals. However, the value of the technique to provide in-depth informa...
3 The high-resolution separation of mAbs facilitated the quantification of charge variants using peak areas. Table 2 summarizes the area percent of charge variants of five consecutive analyses. There was a significant difference in the area percent of the charge variants between two mAbs. The main...