Charge Variant Analysis Protein Aggregate Analysis 滴度(亲和)分析 天然完整蛋白质量分析 Subunit Mass Analysis Meeting your needs for robust charge variant profiling Biotherapeutic proteins such as monoclonal antibodies (mAbs) are far more heterogeneous tha...
Charge Variant Analysis Protein Aggregate Analysis 滴度(亲和)分析 天然完整蛋白质量分析 Subunit Mass Analysis Meeting your needs for robust charge variant profiling Biotherapeutic proteins such as monoclonal antibodies (mAbs) are far more heterogeneous than sma...
In this work, 2D-LC methods for charge variant analysis were developed using a strong cation-exchange chromatography (SCX) as first dimension (1D) separation. Non-porous SCX (3 m) particle columns and different mobile phases were evaluated using a test mixture of some standard proteins of ...
Du et al. Chromatographic Analysis of the Acidic and Basic Species of Recombinant Monoclonal Antibodies. mAbs. 2012 Sep-Oct; 4(5):578–85. Eyer B et al. How Similar Is Biosimilar? A Comparison of Infliximab Therapeutics in Regard to Charge Variant Profile...
This pH gradientmethod serves as a platform method for the mAb charge variant analysis, covering the pH range from 5.6 to 10.2. Most of the therapeutic mAbs have pI values fallingwithin this pH range. Rituxiamab (Figure 1a),... TF Scientific,Sunnyvale,S Lin,... 被引量: 0发表: 2015年...
This pH gradientmethod serves as a platform method for the mAb charge variant analysis, covering the pH range from 5.6 to 10.2. Most of the therapeutic mAbs have pI values fallingwithin this pH range. Rituxiamab (Figure 1a),trastuzumab (Figure 3a), adalimumab (Figure 5a), andbevacizumab (...
Charge variant analysis is a widely used analytical tool in characterization of monoclonal antibodies (mAbs). It depicts the heterogeneity of charge variant forms, some of which may differ by only minor modifications of a single amino acid. The analysis ensures product consistency with no unwanted ...
In this study, we present a robust and reproducible anion exchange mass spectrometry (AEX-MS) method for charge variant analysis using the BioAccord™ LC-MS System equipped with ACQUITY™ Premier UPLC™. Using this method, charged species were chromato...
Imaged capillary isoelectric focusing (icIEF) and ion-exchange chromatography (IEX) are two essential techniques that are routinely used for charge variant analysis of therapeutic monoclonal antibodies (mAbs) during their development and... G Wu,C Yu,W Wang,... 被引量: 0发表: 2023年 High-sensi...
Carboxypeptidase B digestion confirmed that the major basic variant peaks in biosimilar correspond to lysine variants. The Agilent 1260 Infinity Bio-inert Quaternary LC with Bio MAb PEEK columns and reproducible method make this solution particularly suitable for the QA/QC analysis of mAbs for the ...