About 14.8% of the acidic variant can be explained by N-terminal leader sequences in the light chain, and 18% of the acidic variant was demonstrated to be deamidation of asparagine in the heavy chain. There was approximately 54.2% of the acidic variant still cannot be explained. It was ...
We found that the assumption of a linear calibration curve, currently enforced by both icIEF and cIEF ((i)cIEF) analysis software, introduces unpredictable errors in the expected pI-values, across the pH gradient, despite Pearson's determination coefficient being very close to one. We assessed ...
Charge variant mass spectrometryIon exchange chromatographyWeak cation exchangeNative mass spectrometryMolecular modelMispaired antibodyBispecific antibodies (BsAbs) combine the specificities of two antibodies to target different antigens. While production and in vivo assembly of BsAb using a single-cell host...