About 14.8% of the acidic variant can be explained by N-terminal leader sequences in the light chain, and 18% of the acidic variant was demonstrated to be deamidation of asparagine in the heavy chain. There was approximately 54.2% of the acidic variant still cannot be explained. It was ...
Imaged capillary isoelectric focusing (icIEF) and ion-exchange chromatography (IEX) are two essential techniques that are routinely used for charge variant analysis of therapeutic monoclonal antibodies (mAbs) during their development and... G Wu,C Yu,W Wang,... 被引量: 0发表: 2023年 High-sensi...
Quantitative analysis by mass spectrometry is limited to chromatographic separation of the antibody assemblies due to their similar biophysical properties. This is particularly challenging for distinguishing correctly paired and light chain-scrambled mispaired antibodies which have the same molecular weight. ...
文档标签: Imaged Capillary Isoelectric Focusing for Charge 系统标签: icief isoelectric imaged hrge capillary focusing 48BioProcessInternational9(10)November2011 BioProcessTechnical ImagedCapillaryIsoelectric FocusingforCharge-Variant AnalysisofBiopharmaceuticals DavidA.Michels,OscarSalas-Solano,andChantalFelten Produc...
Charge variant mass spectrometryIon exchange chromatographyWeak cation exchangeNative mass spectrometryMolecular modelMispaired antibodyBispecific antibodies (BsAbs) combine the specificities of two antibodies to target different antigens. While production and in vivo assembly of BsAb using a single-cell host...