We investigated the characteristics and functionalities of extracellular vesicles (EVs) from(previously) towards host immune cells..produces EVs that have a cytoplasmic membrane and contain cytoplasmic metaboli
Extracellular vesicles (EVs) are lipid bilayered membrane structures released by all cells. Most EV studies have been performed by using cell lines or body fluids, but the number of studies on tissue-derived EVs is still limited. Here, we present a protocol to isolate up to six different EV...
Raman spectroscopy, especially Raman tweezers microspectroscopy (RTM) is also a candidate for rapid and label-free characterization of cell-derived extracellular vesicles [23], [24], [25]. Infrared (IR) spectroscopy based protein quantification is already commercialized [26] and provide solution for ...
Fungal cells use extracellular vesicles (EVs) to export biologically active molecules to the extracellular space. In this study, we used protoplasts of Aspergillus fumigatus, a major fungal pathogen, as a model to evaluate the role of EV production in cell wall biogenesis. Our results demonstrated...
Techniques used for the isolation and characterization of extracellular vesicles: results of a worldwide survey. J Extracell Vesicles. 2016;5:32945.Gardiner, C.; Di Vizio, D.; Sahoo, S.; Thery, C.; Witwer, K.W.; Wauben, M.; Hill, A.F. Techniques used for the isolation and ...
Extracellular vesiclesMetastatic breast cancerOrganotropismProteomicsDNABiomarkersWhile primary breast cancer (BC) is often effectively managed, metastasis remains the primary cause of BC-related fatalities. Gaps remain in our understanding of the mechanisms regulating cancer cell organotropism with predilection...
Extracellular vesicles (EVs) are secreted by all cells into bodily fluids and play an important role in intercellular communication through the transfer of
has beengiven to the characterization of extracellular vesicles and their components. This review focuses on state-of-the-art proteomic technologies to analyze proteomes of EVs, especially their post-translationalmodif i cations (PTMs). With their strong biological relevance and the relatively non...
Research ArticleLipidomic characterizationof extracellular vesicles in human serumSuming Chen 1 , Amrita Datta-Chaudhuri 1 , Pragney Deme 1 ,Alex Dickens 2 , Raha Dastgheyb 1 , Pavan Bhargava 1 ,Honghao Bi 1 , and Norman J Haughey 1,3AbstractThere is a wide variety of extracellular vesicles...
Extracellular vesicles (EVs), including exosomes, are thought to mediate intercellular communication through the transfer of cargoes from donor to acceptor cells. Occurrence of EV-content delivery within acceptor cells has not been unambiguously demonstrated, let alone quantified, and remains debated. Here...