Totipotency emerges in early embryogenesis, but its molecular underpinnings remain poorly characterized. In the present study, we employed DNA fiber analysis to investigate how pluripotent stem cells are reprogrammed into totipotent-like 2-cell-like cell
Comparing between any two cell types, environmental conditions or species, there are typically thousands of molecular differences such as changes in gene expression, making this a difficult question to answer. We reasoned that one way to address this question would be to ask it in a minimal syste...
However, the sequence and location of TDMRs in the genome were largely unknown. The advent of the mouse and human genome project and virtual RLGS [4], [5], [6] has made it possible to more rapidly identify the DNA sequence of RLGS fragments and the locations of TDMRs [7], [8], ...
Gene expression changes were most pronounced in donors at the disease stage when microscopic LB changes first occur in the sampled brain region. Additionally, we identified disease stage-specific enrichment of brain specific pathways and immune mechanisms. On the contrary, we showed that mitochondrial ...
(Supplementary Table8). In the adult epicardium, we found thatHPwas the best predictive coding gene (F-score = 0.44, precision = 0.3, recall = 0.81) (Fig.4c), followed byHAS1(hyaluronan synthase 1),SLPI(secretory leukocyte peptidase inhibitor),FAM153B(family with sequence ...
The plant growth-hormone auxin (indole-3-acetic acid, IAA) is involved in regulating such diverse processes as cell elongation, cell division and differentiation. The sequence of events leading to the various phenomena is still poorly understood. Both changes in extra- and intracellular pH (refs ...
Transcription factors (TFs) interpret the genome directly, and are responsible for decoding DNA sequences [1]. It is reported that transcriptional factors are key components of cells that control gene expression, determining how the cells function [2]. Transcription factors acting as conductor orchest...
The binding partners of DPO4 in the crystal structures (DNA or PCNA12) are not shown in (C)–(E). DPO4 sequence (y axis) is colored for different domains in (F), with the same color in (C)–(E). The native contacts are determined with Shadow Algorithm Noel et al., 2012, Noel...
structurally similar to ESR1 and ESR212but whose ligands are unknown. ESRRA also has DNA sequence homology to the estrogen receptor and can be activated by phytoestrogens (i.e. genistein)13. These receptors play pivotal roles in metabolism, insulin resistance, NAFLD, and mitochondrial function12. ...
Contemporary single-cell lineage-tracing (scLT) methods use expressed, heritable DNA barcodes to combine cell lineage readout with single-cell transcriptomics. However, reliance on transcriptional profiling limits adaptation to other single-cell assays. With CellTag-multi, we present an approach that ...