IRF3 结构示意图:DNA 结合结构域(DNA binding domain,DBD),干扰素调节因子结合结构域(IRF association domain,IAD),调节结构域(regulatory domain, RD),核定位序列(the nuclear localization sequence,NLS)和出核序列(thenuclear export sequence,NES) IRF家族的 C 端(除IRF1和IRF2外)IAD结构域介导家族成员之间的相...
近日,中国科学技术大学生命科学与医学部/附属第一医院、免疫应答与免疫治疗重点实验室高大兴课题组和中国科学技术大学生命科学与医学部无膜细胞器与细胞动力学教育部重点实验室项晟祺课题组在PNAS杂志上发表了一篇题为Crucial role of the cGAS N te...
domain and two DNA‐binding domains.In the absence of DNA stimulation, cGAS is self‐inhibited.When cGAS recognizes and binds to exogenous DNA derived from dead cells, DNA viruses, retroviruses, bacteria, or endogenous DNA derived from damaged mitochondria or its unstable DNA, it undergoes a ...
cGAS调控因子GTP酶激活蛋白SH3结构域结合蛋白1[GTPase-activating protein-(SH3domain)-binding protein 1,G3BP1]能够增强cGAS和DNA的结合,促进cGAS-DNA复合物的形成。Liu等[35]从绿茶茶多酚中发现的天然小分子表没食子儿茶素没食子酸酯...
1 cGAS-STING 信号通路 自 2012 年陈志坚团队发现 cGAS 是重要的 DNA 识别受体以来,一度成为免疫学研究的热点,并证 实其在自身免疫疾病,肿瘤免疫,癌症以及细胞衰 老等生物学过程中也发挥了重要作用[7].作为核苷 酸转移酶家族成员之一,cGAS 的 N 端能非特异性 地结合 dsDNA 并促进其自身的激活以及决定其在细...
are warranted. cGAS has an obvious DNA binding domain and catalytic pocket, and structure-targeted drugs have also been discovered and developed. In addition, posttranslational modifications such as phosphorylation, acetylation, and glutamylation also regulate cGAS activity, which means that intervention ...
nuclei [26]. To active cGAS, Non-POU domain-containing octamer-binding protein (NONO) first binds to capsid protein DCs nucleus. Then, NONO interacts with cGAS to form a complex which in turn promotes the recognition of HIV-2 DNA and the initiation of nuclear cGAS induced STING activation ...
G3BP1 enhanced DNA binding of cGAS by promoting the formation of large cGAS complexes. G3BP1 deficiency led to inefficient DNA binding by cGAS and inhibited cGAS-dependent interferon (IFN) production. The G3BP1 inhibitor epigallocatechin gallate (EGCG) disrupted existing G3BP1-cGAS complexes and ...
MRE11-binding partners RAD50 double strand break repair protein (RAD50) and nibrin (NBN) — which form the so-called MRE11–RAD50–NBN (MRN) complex — but not MRE11 exonuclease activity, were found to be required for the CGAS stimulation by MRE11, as were MRE11 DNA-binding domains. ...
RD, regulatory domain (light gray). Region around S396 (dark gray). (K–M) Mitotic lifespan of an IRF3 disruption cell line containing rescue constructs expressing doxycycline-inducible WT GFP-IRF3 (K), a mutant lacking the DNA binding domain (L), or a mutant expressing GFP-IRF3 carrying...