Effector (CD44hi, CD62L-) and central (CD44hi, CD62L+) memory T cell subsets in mouse CD3e+ and either CD4+ or CD8a+ splenocytes.Ethan, PoteetPhoebe, LewisFeng, LiSheng, ZhangJianhua, GuChangyi, ChenSam, On HoThai, DoSuMing, Chi...
In unimmunized specific pathogen-free mice, there are unique memory-type CD8(+) T cell populations expressing asialoGM1 (ASGM1). These cells were classified into central memory-type T cells (T(CMT)) judging from their expression profile of CD44, IL-2Rbeta, CD62L and CCR7 cell-surface mo...
Exogenous TGF-β addition to isolated splenocytes from C57BL/6 or T-cells from F5 transgenic mice inhibited the differentiation of CD44+/CD62L+ CM T-cells in a dose-dependent manner. The addition of 0.5 ng/ml of TGF-β reduced the number of CD44+/CD62L+ CM by 50%, independent ...
Phenotypic analysis of T cells stimulated on ICAM-1-null APCs reveals preferential generation of CD44(high) CD62L(low) effector memory cells (T(EM)) over CD44(high) CD62L(high) central memory cells (T(CM)). Further, while the proportion of naive:memory T cells is similar in ...
We found that therapeutic NLGP and CTX treatment generates central memory CD8+ T (TCM) cells with characteristic CD44+CD62LhighCCR7highIL-2high phenotypes. But these TCM cells are functionally impaired to prevent re-appearance of tumors along with compromised proliferative, IL-2 secretive and ...
Figure 1. Activated PI3Kδ T cells show increased cell death, FasL, and pFoxO1 (A) Annexin-V+ T cells from healthy controls and patients with APDS stimulated with anti-CD3 plus anti-CD28 (n = 4). (B) Annexin-V staining of sorted naive (CD62LhiCD44lo) CD8+ cells stimulated with an...
The mechanisms underlying the heightened protection mediated by central memory CD8+ T (TCM) cells remain unclear. Here we show that the transcription factor Tcf1 was required in resting TCM cells to generate secondary effector CD8+ T cells and to clear p
T-cell-derived IFN-γ. Although most early T-cell infiltrates are memory T cells specific for irrelevant antigens, these are replaced by virus-specific T cells, which expand in secondary lymphoid organs and migrate into the CNS parenchyma80. As antiviral T cells accumulate in the CNS, there ...
As shown in Figure 2A, a substantial fraction of effector-memory (CD44high, CD62Llow) CD4+ T cells were increased in the spleen and LN, but not so highly in the thymus, of cKO mice. In addition, we examined cytokine production from the splenic T cells. As s...
Production of effector memory T cells (TEM) before and after intranasal boost immunization in Study 1. At 10 days post-2nd immunization (10 dp2i), spleen samples were collected and subjected to flow cytometry analysis to determine the percentages of CD44+CD62L−TEMcells, CD44+CD62L+TCMcells...