Supplier Promega Kit Type Cell proliferation and viability Detection Method Luminescent View Product On Supplier's Website Add to Procurement List Sign inorregisterto save this reagent to your favourites Supplier provided information Validations
均质分析步骤仅包括直接向培养细胞的血清培养基中加入一种试剂 (CellTiter-Glo™ Reagent) 这一个步骤,不需要洗涤细胞、去除培养基或多步吸量步骤。该系统可在加入试剂并混合后 10 分钟内,检测出 384 孔板上少至 15 个细胞/孔的活细胞。 均质的“加入-混合-测量”形式使得细胞裂解产生的荧光信号与存在的 ATP ...
目的均质方法。其中ATP是细胞代谢活性的指示剂。荧光细胞活性检测是为多孔板应用而设 计,是高通量筛选(HTS)、细胞增殖和毒性分析的理想选择。均质分析步骤仅包括直接向培养 细胞的血清培养基中加入一种试剂(CellTiter-Glo™Reagent)这一个步骤,不需要洗涤细胞、 ...
The system detects as few as 15 cells/well in a 384-well format in 10 minutes after adding reagent and mixing. The homogeneous "add-mix-measure" format results in cell lysis and generation of a luminescent signal proportional to the amount of ATP present. The amount of ATP is directly ...
We next added 100µl of CellTiter-GloTM Reagent, mixed and recorded luminescence. Each experiment was performed in quadruplicate. Experimental wells were compared to a well containing 1µM ATP in 100µl DPBS. (CS, calf serum; HS,horse serum; FBS, fetal bovine serum)....
The experiment is quite simple. First, cells are seeded in a 96 well plate with 1 to 5,000 cells per well. Second, cells are treated with the experimental drug for 2 to 3 days. Third, the detection reagent is prepared by combining the CellTiter Glo® Buffer with CellTiter Glo® Subs...
We next added 100µl of CellTiter-GloTM Reagent, mixed and recorded luminescence. Each experiment was performed in quadruplicate. Experimental wells were compared to a well containing 1µM ATP in 100µl DPBS. (CS, calf serum; HS,horse serum; FBS, fetal bovine...
.promega TM403 · Revised 3/15 F 6 8 4 7 M B CellTiter-Glo ® 2.0 Reagent Mix Luminometer igure 2. Overview of the CellTiter-Glo ® 2.0 Assay protocol. The luci erase reaction or this assay is shown in Figure 3. The “add-mix-measure” ormat results in cell lysis and ...
Reagent. Promega Corporation ·2800 Woods Hollow Road ·Madison, WI 53711-5399 USA Toll F ree in USA 800-356-9526·Phone 608-274-4330 ·F ax 608-277-2516 ·www.promega.com 3170M A 12_0A CellTiter-Glo CellTiter-Glo Mixer Luminometer ...
.promega 1. Description (continued) 3170MA12_0A CellTiter-Glo® Substrate CellTiter-Glo® Reagent Mix Luminometer CellTiter-Glo® Buffer Figure 1. Flow diagram showing preparation and use of CellTiter-Glo® Reagent. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA...