均质分析步骤仅包括直接向培养细胞的血清培养基中加入一种试剂 (CellTiter-Glo™ Reagent) 这一个步骤 不需要洗涤细胞、去除培养基或多步吸量步骤。该系统可在加入试剂并混合后 10 分钟内 检测出 384 孔板上少至 15 个细胞/孔的活细胞。 均质的“加入-混合-测量”形式使得细胞裂解产生的荧光信号与存在的 ATP ...
目的均质方法。其中ATP是细胞代谢活性的指示剂。荧光细胞活性检测是为多孔板应用而设 计,是高通量筛选(HTS)、细胞增殖和毒性分析的理想选择。均质分析步骤仅包括直接向培养 细胞的血清培养基中加入一种试剂(CellTiter-Glo™Reagent)这一个步骤,不需要洗涤细胞、 ...
Kit Type Cell proliferation and viability Detection Method Luminescent View Product On Supplier's Website Add to Procurement List Sign inorregisterto save this reagent to your favourites Supplier provided information Validations None provided Reactivity ...
When using the Beckman Coulter Biomek(R) FX orHamilton MICROLAB(R) STAR workstation, 100ml of reagent is sufficient to perform 384 x 100µl assays in 96-wellplates or 1,536 x 25µl assays in 384-well plates. The single-plate Biomek(R) 2000 metho...
2) 将白色 瓶中 100 mL 的 CellTiter-Glo Buffer 完全转移至棕色 瓶中,与 CellTiter-Glo Substrate 配制成CellTiter-Glo Reagent 。充分混匀后可立即使用。可 根据每次实验的用量分装CellTiter-Glo Reagent ,小包装-20 ℃保存,避免反复冻 融,使用前平衡至室温并混匀。 3) 细胞板平衡至室温后,每孔中加入与...
Reagent. Promega Corporation ·2800 Woods Hollow Road ·Madison, WI 53711-5399 USA Toll F ree in USA 800-356-9526·Phone 608-274-4330 ·F ax 608-277-2516 ·www.promega.com 3170M A 12_0A CellTiter-Glo CellTiter-Glo Mixer Luminometer ...
The system detects as few as 15 cells/well in a 384-well format in 10 minutes after adding reagent and mixing. The homogeneous "add-mix-measure" format results in cell lysis and generation of a luminescent signal proportional to the amount of ATP present. The amount of ATP is directly ...
Reagent and CellTiter- Glo ® 2.0 Reagent were incubated in a 22°C water bath or di erent lengths o time and then rozen at –80°C. A ter thawing, the reagent samples were mixed with an equal volume o 2μM ATP in water, and the luminescence was ...
Briefly, the CellTiter-Glo® Reagent is added directly to the wells of the plate (100 μL per ... CS Atwood,SV Meethal - US 被引量: 4发表: 2010年 Biological Evaluation of Two 1,4-Naphthoquinone Derivatives Against a Breast Human Adenocarcinoma Cell Line effect was assayed against MDA-...
The experiment is quite simple. First, cells are seeded in a 96 well plate with 1 to 5,000 cells per well. Second, cells are treated with the experimental drug for 2 to 3 days. Third, the detection reagent is prepared by combining the CellTiter Glo® Buffer with CellTiter Glo® Subs...