均质分析步骤仅包括直接向培养细胞的血清培养基中加入一种试剂 (CellTiter-Glo™ Reagent) 这一个步骤 不需要洗涤细胞、去除培养基或多步吸量步骤。该系统可在加入试剂并混合后 10 分钟内 检测出 384 孔板上少至 15 个细胞/孔的活细胞。 均质的“加入-混合-测量”形式使得细胞裂解产生的荧光信号与存在的 ATP ...
细胞的血清培养基中加入一种试剂(CellTiter-Glo™Reagent)这一个步骤,不需要洗涤细胞、 去除培养基或多步吸量步骤。该系统可在加入试剂并混合后10分钟内,检测出384孔板上少 至15个细胞/孔的活细胞。 均质的“加入-混合-测量”形式使得细胞裂解产生的荧光信号与存在的ATP量成正比。与 ...
Cell proliferation and viability Detection Method Luminescent View Product On Supplier's Website Add to Procurement List Sign inorregisterto save this reagent to your favourites Supplier provided information Validations None provided Reactivity None provided ...
Automated CellTiter-Glo® Luminescent Cell Viability Assay ProtocolEach 100ml of reagent is sufficient to perform 768 x 100µl assays in 96-well plates or 3,072 x 25µl assays in 384-well plates using the Beckman Coulter Biomek(R) 2000 workstation. W...
The CellTiter-Glo® Assay generates a "glow-type" luminescent signal, which has a half-life generally greater than five hours, depending on cell type and medium used. The extended half-life eliminates the need to use reagent injectors and provides flexibility for continuous or batch mode ...
Figure 1. Flow diagram showing preparation and use of CellTiter-Glo ? Reagent. Promega Corporation ·2800 Woods Hollow Road ·Madison, WI 53711-5399 USA Toll F ree in USA 800-356-9526·Phone 608-274-4330 ·F ax 608-277-2516 ·www.promega.com ...
干粉状CellTiter-Glo Substrate 同样在 使用前平衡至室温。 2) 将白色 瓶中 100 mL 的 CellTiter-Glo Buffer 完全转移至棕色 瓶中,与 CellTiter-Glo Substrate 配制成CellTiter-Glo Reagent 。充分混匀后可立即使用。可 根据每次实验的用量分装CellTiter-Glo Reagent ,小包装-20 ℃保存,避免反复冻 融,使用前平衡...
The CellTiter-Glo ® 2.0 Assay is designed or use with multiwell-plate ormats, making it ideal or automated high- throughput screening (HTS) and cell proli eration and cytotoxicity assays. The homogeneous assay procedure (Figure 2) involves addition o a single reagent (CellTiter-Glo ...
The number of viable cells attached to the plate is determined using the CellTiter-Glo® Luminescent Cell Viability Assay (Promega). Briefly, the CellTiter-Glo® Reagent is added directly to the wells of the plate (100 μL per ... CS Atwood,SV Meethal - US 被引量: 4发表: 2010年 ...
an equal volume of CellTiter-Glo® Reagent is added to each well of cell culture medium (you do not need to change medium). I used 100 ul in each well of a 96 well plate. After mixing for 2 minutes, the plate sits at room temperature for 10 minutes to stabilize the luminescence. ...