均质分析步骤仅包括直接向培养细胞的血清培养基中加入一种试剂 (CellTiter-Glo™ Reagent) 这一个步骤,不需要洗涤细胞、去除培养基或多步吸量步骤。该系统可在加入试剂并混合后 10 分钟内,检测出 384 孔板上少至 15 个细胞/孔的活细胞。 均质的“加入-混合-测量”形式使得细胞裂解产生的荧光信号与存在的 ATP ...
Promega Kit Type Cell proliferation and viability Detection Method Luminescent View Product On Supplier's Website Add to Procurement List Sign inorregisterto save this reagent to your favourites Supplier provided information Validations None provided ...
细胞的血清培养基中加入一种试剂(CellTiter-Glo™Reagent)这一个步骤,不需要洗涤细胞、 去除培养基或多步吸量步骤。该系统可在加入试剂并混合后10分钟内,检测出384孔板上少 至15个细胞/孔的活细胞。 均质的“加入-混合-测量”形式使得细胞裂解产生的荧光信号与存在的ATP量成正比。与 ...
The CellTiter-Glo® Assay generates a "glow-type" luminescent signal, which has a half-life generally greater than five hours, depending on cell type and medium used. The extended half-life eliminates the need to use reagent injectors and provides flexibility for continuous or batch mode ...
Reagent. Promega Corporation ·2800 Woods Hollow Road ·Madison, WI 53711-5399 USA Toll F ree in USA 800-356-9526·Phone 608-274-4330 ·F ax 608-277-2516 ·www.promega.com 3170M A 12_0A CellTiter-Glo CellTiter-Glo Mixer Luminometer ...
The experiment is quite simple. First, cells are seeded in a 96 well plate with 1 to 5,000 cells per well. Second, cells are treated with the experimental drug for 2 to 3 days. Third, the detection reagent is prepared by combining the CellTiter Glo® Buffer with CellTiter Glo® Subs...
We next added 100µl of CellTiter-GloTM Reagent, mixed and recorded luminescence. Each experiment was performed in quadruplicate. Experimental wells were compared to a well containing 1µM ATP in 100µl DPBS. (CS, calf serum; HS,horse serum; FBS, fetal bovine...
The number of viable cells attached to the plate is determined using the CellTiter-Glo® Luminescent Cell Viability Assay (Promega). Briefly, the CellTiter-Glo® Reagent is added directly to the wells of the plate (100 μL per ... CS Atwood,SV Meethal - US 被引量: 4发表: 2010年 ...
.promega TM403 · Revised 3/15 F 6 8 4 7 M B CellTiter-Glo ® 2.0 Reagent Mix Luminometer igure 2. Overview of the CellTiter-Glo ® 2.0 Assay protocol. The luci erase reaction or this assay is shown in Figure 3. The “add-mix-measure” ormat results in cell lysis and ...
干粉状CellTiter-Glo Substrate 同样在 使用前平衡至室温。 2) 将白色 瓶中 100 mL 的 CellTiter-Glo Buffer 完全转移至棕色 瓶中,与 CellTiter-Glo Substrate 配制成CellTiter-Glo Reagent 。充分混匀后可立即使用。可 根据每次实验的用量分装CellTiter-Glo Reagent ,小包装-20 ℃保存,避免反复冻 融,使用前平衡...