Cell-derived matrices (CDM)—Methods, challenges and applications - ScienceDirectBook_ChapterExtracellular matrix (ECM) provides both physical support and bioactive signals such as growth factors and cytokines to cells at their microenvironment or niche. Engineering the matrix niche becomes an important ...
This protocol describes how to produce cell-derived matrices from fibroblasts. These matrices can be used to provide a 3D scaffold for cell culture and to investigate cell behavior in complex microenvironments. 2D surfaces offer simple analysis of cells
Cell-derived matrices (CDMs) offer a more physiologically relevant alternative for studying in vivo-like cell behavior in vitro. In the protocol described here, fibroblasts cultured on gelatin-coated surfaces are maintained in the presence of ascorbic acid to strengthen matrix deposition over 1-3 ...
Cell-derived matrices (CDM)—Methods, challenges and applications - ScienceDirect 来自 Elsevier 喜欢 0 阅读量: 27 作者:H.W. Cheng ,M.T. Yuan ,C.W. Li ,B.P. Chan 摘要: Extracellular matrix (ECM) provides both physical support and bioactive signals such as growth factors and cytokines to...
Cell-Derived Matrices, Part A, Volume 156,provides a detailed description and step-by-step methods surrounding the use of three-dimensional cell-derived matrices for tissue engineering applications. Biochemical, biophysical and cell biological approaches are presented, along with sample results. Specific...
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This chapter provides a step-by-step method for producing multilayered (e.g., 3D) fibroblastic cell-derived matrices (fCDM). Methods also include means to assess ECM topography and other cellular traits, indicative of fibroblastic functional statuses, like naïve/normal vs. inflammatory and/or...
Extracellular microparticles (MPs) can function as drug-delivery vehicles for anticancer drugs. Here, we show that the softness of MPs derived from tumour-repopulating cells (TRCs) isolated from three-dimensional fibrin gels enhances the MPs’ drug-deliv
Cukierman, E. (2005). Cell Migration Analyses Within Fibroblast-Derived 3-D Matrices. In: Guan, JL. (eds) Cell Migration. Methods in Molecular Biology™, vol 294. Humana Press. https://doi.org/10.1385/1-59259-860-9:079 Download citation ...
Ovarian stiffness increases with age in the mammalian ovary and depends on collagen and hyaluronan matrices. Aging Cell. 2020;19(11):e13259. Article CAS PubMed PubMed Central Google Scholar Verschoor CP, Johnstone J, Millar J, Dorrington MG, Habibagahi M, Lelic A, et al. Blood CD33(+)...