However, it is difficult to prepare matrices mimicking native ECM using chemical methods because the ECM has a complicated composition. The decellularization technique is useful to prepare such matrices. In this chapter, we described the protocol to prepare matrices mimicking native ECM surrounding ...
derived matrices with controlled 3D architecture, Generation of functional fluorescently-labelled cell-derived matrices by means of genetically-modified fibroblasts, Bi-layered cell-derived matrices, Engineering clinically-relevant cell-derived matrices using primary fibroblasts, Decellularized matrices for ...
The development and application of decellularized extracellular matrices (ECM) has grown rapidly in the fields of cell biology, tissue engineering and regenerative medicine in recent years. Similar to decellularized tissues and whole organs, cell-derived matrices (CDM) represent bioactive, biocompatible ma...
select article Chapter 5 - Cell-derived decellularized extracellular matrices Book chapterAbstract only Chapter 5 - Cell-derived decellularized extracellular matrices Greg M. Harris, Irene Raitman, Jean E. Schwarzbauer Pages 97-114 Purchase PDF Chapter preview select article Chapter 6 - Analysis of ...
scaffold-based and scaffold-free approaches can be used. Scaffolds for cardiac cell therapy include decellularized matrices, injectable biomaterials, and cardiac patches made of synthetic or natural hydrogels.163,164Scaffold-free tissue-based constructs such as cell sheets and microtissues also exist.165...
Cultured cell-derived decellularized extracellular matrix (cultured cell-derived dECM): Future applications and problems — a mini review Takashi Hoshiba, in Current Opinion in Biomedical Engineering, 2021 Cancer cells dECM has been applied for cancer cell culture as well as the cultures of normal ...
It was found that mature absorptive cells, cupped cells, and various intestinal endocrine cells could be detected in intestinal epithelial organoids derived from Lgr5(+) CBC [36,117]. These cells have corresponding physiological functions such as transporting substances, absorbing nutrients, and synthe...
Numerous studies have shown that compared with other biomaterials, decellularized extracellular matrices (ECMs) can provide a good microenvironment for cell growth and are more beneficial for the repair of joint defects. Low-temperature-deposited 3D-printed decellularized chondrocyte ECM scaffolds have ...
By applying a new chemical process, human cartilage tissues can be decellularized and sterilized, and these decellularized matrices can be used as chondro-inductive materials for cartilage replacement [24]. Compared with tissue-derived DECM, cell-derived DECM has recently gained a lot of interest ...
Decellularized tissue and cell-derived extracellular matrices as scaffolds for orthopaedic tissue engineering Biotechnol. Adv., 32 (2014), pp. 462-484, 10.1016/j.biotechadv.2013.12.012 View PDFView articleView in ScopusGoogle Scholar [119] F.M. Chen, X. Liu Advancing biomaterials of human origi...