今年 4 月,Nature Cell Biology 发表最新研究:通过 Seahorse 能量代谢分析,揭示靶向 SHP-1 调控白血病干细胞(LSC)的能量代谢,可有效克服急性髓性白血病中的 LSC 耐药 [2]。 体外代谢分析提供了识别与干细胞分化相关的关键代谢变化的信息,从而能够预测细胞功能,揭示可能的重编程和分化潜能,帮助优化疾病研究进程。那么...
The crystal structure of ZK53/ClpP complex reveals a π-π stacking effect that is essential for ligand binding selectively to the mitochondrial ClpP. ZK53 features on a simple scaffold, which is distinct from the activators with rigid scaffolds, such as acyldepsipeptides and imipridones. ZK53...
3b, c, g). Both MIF and MK were also upregulated on protein level in NB tumors and cell lines compared to controls, and receptor expression was high on resting and activated monocytes (Fig. 3h, i). To experimentally test the interactions of NB with myeloid cells, we measured secreted ...
OPA1 has a central role in regulating not only mitochondrial shape but also mitochondrial cristae structure that, on one side, controls apoptosis (through cytochrome c sequestration or release) and, on the other side, metabolism (by favoring the quaternary organization of ETC subunits in super...
3). Therefore, in obese adipose tissue, O-ADSCs may play a crucial role in activating and maintaining M1-type macrophages, which could contribute to the development and maintenance of obesity and obesity-related diseases. Intriguingly, we found that the mitochondrial structure of O-ADSCs was ...
The structure and the functions of ER-mitochondria interface are regulated by several proteins located in ER and/or mitochondrial membranes, particularly at the level of the so called mitochondria associated membranes (MAM; [21]). Here, key steps of processes, such as modulation of intracellular ...
复旦大学附属肿瘤医院雷群英课题组在Nature Cell Biology杂志上发表了题为 “BCAT2-mediated BCAA catabolism is critical for development of pancreatic ductal adenocarcinoma” 的研究论文。作者揭示了BCAA - …
RSL3不影响OCR,这与之前的报道一致,表明线粒体在RSL3诱导的铁死亡机制中是不可缺少的。PC(22:6, 22:6)处理的细胞在检测过程中没有改变形态,这表明观察到的OCR降低是由于抑制线粒体呼吸而不是细胞活力(图S5B)。此外,还评估了其他ETC复合物抑制剂,包括复合物I抑制剂鱼藤酮和复合物III抑制剂抗霉素a。这些抑制...
a, Schematic of the scCRISPR screening strategy.b, Relative ratio (log2(fold-change (FC))) of cells with gene-level perturbation compared with sgNTC. Vertical line, TFs excluded for initial network analyses.c, Co-functional modules (with the six major modules highlighted in red) and co-reg...
1b,c). Additionally, we observed that the enhanced growth of the tumour organoids was not due to the incorporation of HPS cells within the organoid structure (Extended Data Fig. 1d). To identify the CAF-derived metabolites regulating cancer cell growth, we performed NMR-based metabolomics to ...