In a simplified view, complexes I, III, and IV transport protons from the mitochondrial matrix into the intermembrane space (between the IMM and the outer mitochondrial membrane [OMM]) in specific invaginations of the IMM called cristae. The mitochondrial ATP synthase (often called complex V) ...
今年 4 月,Nature Cell Biology 发表最新研究:通过 Seahorse 能量代谢分析,揭示靶向 SHP-1 调控白血病干细胞(LSC)的能量代谢,可有效克服急性髓性白血病中的 LSC 耐药 [2]。 体外代谢分析提供了识别与干细胞分化相关的关键代谢变化的信息,从而能够预测细胞功能,揭示可能的重编程和分化潜能,帮助优化疾病研究进程。那么...
test the interactions of NB with myeloid cells, we measured secreted levels of MIF and MK proteins in the supernatants of NB cell lines, peripheral blood mononuclear cells (PBMCs), and co-cultures of NB cells with PBMCs, either in direct contact or through a trans-well membrane. Levels...
a, PDHc activity of CD8+T cells treated with DEG for 30 min (acute) or 7 days (chronic), normalized to total protein concentration. Two-tailed, pairedt-test; acute,n = 9; chronic,n = 14.b, Basal ECAR in CD8+T cells as determined by Seahorse analysis after 30-min DEG ...
Indeed, Ca2+, a key intracellular second messenger, can reach the mitochondrial Inter-Membrane Space (IMS) and then the matrix, where it regulates proteins, enzymes and transporters responsible for ATP synthesis [1]. In turn, this latter is essential for the modulation of several cellular ...
4d). At the same time, the protein level of translocase of outer mitochondrial membrane Tom20 was lower in O-ADSCs than that in L-ADSCs (Fig. 4e and Additional file 2: Fig S2). Fig. 4 Mitochondrial morphology and function of L-ADSCs and O-ADSCs. a Representation of TEM images of...
As a consequence, CSCs exhibit increased mitochondrial mass and membrane potential (reflection of mitochondrial function) [14]. While OXPHOS involves a significantly greater number of biochemical reactions, it is almost 20 times more efficient than glycolysis in terms of generation of ATP per unit of...
复旦大学附属肿瘤医院雷群英课题组在Nature Cell Biology杂志上发表了题为 “BCAT2-mediated BCAA catabolism is critical for development of pancreatic ductal adenocarcinoma” 的研究论文。作者揭示了BCAA - …
c Effect of ZK53 on the oxygen consumption rate (OCR) in H1703 and H520 cells using Seahorse Analyzer (n = 6 biological samples). Rot rotenone, AA antimycin A. Effect of ZK53 on the mitochondrial membrane potential (d), ATP level (e), and mitochondrial ROS (f) in H1703 and ...
State Key Laboratory of Membrane Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, China Jun Xia & Feng Liu Center for Epigenomics, Department of Cellular and Molecular Medicine, University of California, San Diego, School of Medicine, La Jolla, CA, USA ...