Samples containing equivalent amounts of protein (40–80 μg) were loaded and separated on 10–12.5% SDS-PAGE gels and subsequently transferred to PVDF membranes (Millipore). These membranes were blocked for 1 h at room temperature with 5% non-fat dry milk in TBS containing 0.1% Tween ...
Afterwards, the mixture was passed through nitrocellulose membranes (0.45 μM, Schleicher and Schuell Biosciences GmbH, Dassel, Germany) and washed with 800 μl binding buffer w/o serum. The amount of radioactively labelled ssODNs bound to protein was detected with a FLA-3000 (...
Since the late 1990s, this simple view of lipids as solvents for proteins has been overcome and a more complex picture has been accepted. Thus, as result of an asymmetrical distribution of specific lipids, membranes are further organized in lipid rafts (LRs), defined by being detergent-...
Since MIA PaCa-2 cells almost exclusively expressed PMCA4 at both the protein and mRNA level, this suggests that MIA PaCa-2 represents a good cellular model of PMCA4-overexpressing PDAC that can be taken forward for further functional studies. Moreover, as our previous studies by James, A.D...
Presented a cross- flow filtration chip for extracting plasma from whole blood, which includes a cross- flow layer, a Ni-Pd alloy micro-porous membrane and a reservoir layer, shown in Figure 6b. The comparative experiments were completed using various diluted blood samples, membranes with ...
These antimicrobials are thought to interact with thiol, carboxyl, hydroxyl, amino, phosphate, and imidazole groups that are located on bacterial membranes, leading to membrane deformations and cell wall collapse [35]. As a result, entry points into the bacterial cells appear, allowing for further...
Moreover, some annexins have high affinity for highly curved membranes that appear at free edges near rupture sites, a property that might accelerate their recruitment for rapid repair. Here, we discuss the mechanisms of annexin-mediated membrane shaping and curvature sensing in the light of our ...
CF4 plasma surface modification of asymmetric hydrophilic polyethersulfone membranes for direct contact membrane distillation. J. Membr. Sci. 2012, 407, 164–175. [CrossRef] 41. Mattioli, S.; Martino, S.; D'Angelo, F.; Emiliani, C.; Kenny, J.M.; Armentano, I. Nanostructured Polystyrene ...
International Journal of Molecular Sciences Article Glycosylation Modulates Plasma Membrane Trafficking of CD24 in Breast Cancer Cells Amanda Chantziou 1, Kostas Theodorakis 2, Hara Polioudaki 1, Eelco de Bree 3 , Marilena Kampa 4, Dimitris Mavroudis 5,6, Elias Castanas 4 and Panayiotis A. ...
International Journal of Molecular Sciences Article Platelet-Rich Plasma Increases the Levels of Catabolic Molecules and Cellular Dedifferentiation in the Meniscus of a Rabbit Model Hye-Rim Lee 1, Oog-Jin Shon 2, Se-Il Park 3, Han-Jun Kim 1, Sukyoung Kim 4, Myun-Whan Ahn 2 and Sun Hee ...