According to this theory, the formation of a nucleus of “crystallization” within the cytoblast was the first phase of cell generation. Subsequent to nucleus formation was the enlargement of condensed material leading to the formation of a new cell [3]. This theory of “free cell formation”...
The resource, which is also provided as an interactive web portal, includes 11.3M human cells from 14 main regions and 30 subregions of the brain (Supplementary Fig.1), while the mouse data include 15M cells. Single-cell RNA sequencing and single-nucleus RNA sequencing data of the brain wer...
Our view of eukaryotic chromosomes is still very much dictated by the classic ideas of geneticists and cytologists considering the chromosome just as a vehicle for genes. This one-sided view of chromosomes may have been strongly influenced by the many cytological observations made on polytene ...
Zn2+ is required for the activity of many mitochondrial proteins, which regulate mitochondrial dynamics, apoptosis and mitophagy. However, it is not unders
Cell Nucleus Transplantation In subject area: Immunology and Microbiology Nuclear transfer is a term used to describe the basic technique that involves the transfer of a nucleus from one cell to another cell from which the nucleus has been removed. From: Current Therapy in Large Animal Theriogenolog...
The nuclear proteins, which include structural proteins of the matrix and lamina, RNA and DNA polymerases, and gene regulatory proteins, are synthesized in the cytoplasm and brought into the nucleus. Thus, nuclear proteins must pass the double-membrane barrier of the nuclear envelope. The transport...
(d) qRT‒PCR detection of the expression of circHERC1 and its parent gene after circHERC1 overexpression or knockdown. ***p < 0.001. (e, f) qRT‒PCR analysis of circHERC1, GAPDH, and U6 in the cytoplasm and nucleus in NCI-H3255 cells. (g) FISH assay showed that circHERC1...
During mouse embryonic development, pluripotent cells rapidly divide and diversify, yet the regulatory programs that define the cell repertoire for each organ remain ill-defined. To delineate comprehensive chromatin landscapes during early organogenesis,
Totipotency emerges in early embryogenesis, but its molecular underpinnings remain poorly characterized. In the present study, we employed DNA fiber analysis to investigate how pluripotent stem cells are reprogrammed into totipotent-like 2-cell-like cell
(Crick1958,1970). DNA is transcribed into RNA, which becomes translated into proteins. The first step of gene expression, transcription, occurs in the cell nucleus assisted by the intricate interplay of molecular machinery that acts on chromatin, the functional form of DNA inside cells (Van ...