Diffuse and granular staining for Dnmt1 was exclusively observed in the nucleus of cells in interphase, and cells in metaphase showed localization of Dnmt1 in chromosomes. The expression of Dnmt3a was similar to that of Dnmt1; however, a few cells displayed granular cytoplasmic stainability as ...
Metaphase II-arrested oocytes were collected from superovulated F1 female mice (C57Bl/6J × CBA) aged <10 weeks and cumulus cells were removed using hyaluronidase. Oocytes were enucleated in a droplet of M2 medium containing 5 μg ml−1 of cytochalasin B (CB) using a blunt ...
Since these phosphorylation events do not occur in G1, S, or G2 and are reduced greatly in metaphase, it is concluded that these two specific phosphorylation events are involved with condensation of interphase chromatin into mitotic chromosomes. This conclusion is supported by loss of prelabeled ...
Cells vary in width from about 15 µm to about 25 µm. The stages are (from top left): interphase cortical microtubule array; pre-prophase band of microtubules (predicts future plane of division); metaphase mitotic spindle; telophase, showing early phragmoplast and cell plate; fully ...
Using its intensity as a reference, we estimate that metaphase cells recruit, on average, at least 70 Kar9 molecules at the tip of a single microtubule. Fig. 6: Kar9 stoichiometry at microtubule plus-ends. a, Micrographs showing Dam1-mNeonGreen or Kar9-mNeonGreen localization in haploid ...
The heterochromatin of the haploid sperm nucleus (SN) is highly compacted by protamines and the diploid egg nucleus (EN) is arrested in metaphase of the second meiotic division. (2) At fertilization, factors in the egg cytoplasm remodel the chromatin of the sperm pronucleus (SPN) so that ...
CytoTrack Dyes allow you to measure cell proliferation in live cells. As the labeled cells divide, the concentration of the dye is halved and the proliferation can be measured based upon the varying levels of fluorescence in subsequent generations. ...
In each differentiation assay, cells grown in the regular medium were used as the negative control. Karyotype analysis Karyotyping was conducted using the Giemsa stain technique. First, cell division was halted in metaphase with 0.3 µg/mL colchicine (Solarbio, China) at 37 °C for 2–3 h....
Chromosomes whose origin could not be determined are labeled ‘nd’. (C) Chromosome numbers in metaphases from 145 S2R + cells. (D) A heatmap summarizing chromosome numbers. Metaphase spreads for all the cell lines are provided in Additional file 1. Full size image...
Ploidy status in K-HME cells. A. Metaphase chromosome spread hybridized with X (red; arrowheads) and 17 (green; arrows) centromere probes. B. Interphase cells hybridized with X (red) and 17 (green) centromere probes. The majority (96.9%) of the established breast epithelial cells are diploi...