Cyclin A is destroyed in prometaphase and can delay chromosome alignment and anaphase. J. Cell Biol. 153, 121–136 (2001). Google Scholar Yamano, H. APC/C: current understanding and future perspectives. F1000Res. https://doi.org/10.12688/f1000research.18582.1 (2019). Article PubMed Pub...
Metaphase II-arrested oocytes were collected from superovulated F1 female mice (C57Bl/6J × CBA) aged <10 weeks and cumulus cells were removed using hyaluronidase. Oocytes were enucleated in a droplet of M2 medium containing 5 μg ml−1 of cytochalasin B (CB) using a blunt ...
Human embryonic lung cells, MRC-5, grown in Dulbecco's medium were exposed to various concentrations of the dust extracts of pesticide-free (untreated) beech, oak and pine woods. Three concentrations per extract with and without metabolic activation (S9) and 100 metaphase cells per dose were ...
4、phaseMitosis Double-chromatid chromosomes evident Chromatin becomes super-coiled & compact Nuclear envelop breaks down Nucleoli disappear in onion root tip Centrioles migrate toward poles of cell forming the spindle Double-chromatid chromosomes Onion root tipWhitefish blastulaMetaphase Mitosis Doubl 5、...
(prometaphase). At this point, kinetochores that are unattached to the mitotic spindles generate a “wait” signal that delays the onset of anaphase until all chromosomes are properly attached and aligned. This signal is also called mitotic checkpoint or spindle assembly checkpoint (SAC), which ...
Lamin B remains associated with remnant membrane vesicles when phosphorylated, whereas lamins A and C are dispersed within the prometaphase cell. Coincident with the decondensation of chromosomes in daughter cells (telophase), the phosphates are removed from the lamin proteins by an activated lamin ...
[41] identified multiple subpopulations of cells in each region in multiple regions of human metaphase embryos by single-cell sequencing techniques, and analyzed gene expression and neuronal maturation in these regions. Zhong et al. [42] mapped a single-cell transcriptome map of human prefrontal ...
The [Ca 2+ ] i transients are correlated with cell-cycle events: pronuclear migration, nuclear envelope breakdown, the metaphase–anaphase transition of mitosis and cytokinesis. Mitotic events can be induced by injecting calcium and prevented by injecting calcium chelators into the sea urchin embryo...
Some metaphases show apoptotic morphology electron-microscopically. These results suggest that the testes of MRL strains will provide a useful model for the study of the mechanism of metaphase-specific apoptosis in meiotic spermatocytes. 展开 关键词:...
From diplotene through metaphase, bivalent methylation/phosphorylation post-translational modifications will be imposed, namely: H3K9me2/H3S10ph and H3K27me2/H3S28ph [199]. Those modifications could facilitate specific condensed chromatin conformation during meiosis, as well as mitosis [199]. Addition...